We narrowed to 2,376 results for: pET28
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Plasmid#227213PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationL299A; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
V485G USP7 catalytic
Plasmid#227209PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationV485G; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
P273H USP7 catalytic
Plasmid#227208PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationP273H; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Y331H USP7 catalytic
Plasmid#227205PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationY331H; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
M225I USP7 catalytic
Plasmid#227206PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationM225I; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 N418S catalytic
Plasmid#227202PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationN418Sl; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
K420E USP7 catalytic
Plasmid#227210PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationK420E; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 G392D catalytic
Plasmid#227203PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationG392D; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
E345K USP7 catalytic
Plasmid#227211PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationE345K; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L373F USP7 catalytic
Plasmid#227207PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationL373F; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
G392A USP7 catalytic
Plasmid#227204PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationG392A; aa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CpX-1
Plasmid#218676PurposeExpresses complexin-1 in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
STREP-SUMO-FLAG-SeKaiC
Plasmid#218179PurposeWT SeKaiC for phosphorylation/dephosphorylation studyDepositorInsertSTREP-SUMO-FLAG-SeKaiC
TagsSTREP-TagExpressionBacterialAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMJ074
Plasmid#215654PurposepET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143DepositorInsertYellow fluorescent protein
UseSynthetic BiologyTags10x histidine tagExpressionBacterialPromoterT7Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL28_Cas9-His Δcys E532C/E1207C
Plasmid#206289PurposeBacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574S, E1207CPromoterT7Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL25_Cas9-His Δcys E1207C
Plasmid#206286PurposeBacterial expression of Cas9-His Δcys E1027C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1207CPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL23_Cas9-His Δcys E945C
Plasmid#206284PurposeBacterial expression of Cas9-His Δcys E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E945CPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL24_Cas9-His Δcys E1026C
Plasmid#206285PurposeBacterial expression of Cas9-His Δcys E1026C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1026CPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL27_Cas9-His Δcys E532C/E945C
Plasmid#206288PurposeBacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574S, E945CPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL26_Cas9-His Δcys M1C/E532C
Plasmid#206287PurposeBacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, E532C, C574SPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL22_Cas9-His Δcys E532C
Plasmid#206283PurposeBacterial expression of Cas9-His Δcys E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574SPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO5(wt)
Plasmid#191803PurposeFull length wt AtPCO5 with Nt-His tagDepositorInsertAtPCO5
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO1(wt)
Plasmid#191798PurposeFull length wt AtPCO1 with Nt-His tagDepositorInsertAtPCO1
Tags6xHis, Thrombin cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO2(wt)
Plasmid#191799PurposeFull length wt AtPCO2 with Nt-His tagDepositorInsertAtPCO2
Tags6xHis, Thrombin cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO3(wt)
Plasmid#191800PurposeFull length wt AtPCO3 with Nt-His tagDepositorInsertAtPCO3
Tags6xHis, Thrombin cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK C-term
Plasmid#186147PurposeFor expresseion of recombinant FAK c-terminal domain in E. coliDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH858
Plasmid#172176PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
ExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH859
Plasmid#172177PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
ExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH768v2
Plasmid#172173PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2]
ExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH769v2
Plasmid#172174PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
ExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pThuAsol
Plasmid#171675PurposepThuAsol expression in E. coliDepositorInsertSUMO-TEV-ThuAsol
TagsHis-tag and SUMO-tagExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-2
Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
4E1H.95_LUMABS
Plasmid#155208PurposeExpresses 4E1H.95_LUMABS sensor in E.coliDepositorInsert4E1H.95_LUMABS
Tags6x His TagAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK130
Plasmid#71129PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
TagsHis6ExpressionBacterialMutationchanged valine-319 to phenylalaninePromoterT7Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS09
Plasmid#52649Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS09
ExpressionMammalianMutationL414T mutated from GCaMP3PromoterT7Available SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS05
Plasmid#52646Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS05
ExpressionMammalianMutationM373Q mutated from GCaMP3PromoterT7Available SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS08
Plasmid#52648Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS08
ExpressionMammalianMutationT412Q/L414T mutated from GCaMP3PromoterT7Available SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
p40deltaPX (w/out aa1-135)
Plasmid#19019DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pDrSR beta deltaN
Plasmid#13730DepositorInsertSignal recognition particle receptor β subunit
Tags6xHisExpressionBacterialMutationexpresses residues 60-266 of Danio rerio SRβ with…Available SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMIIS793
Plasmid#238206PurposeExpresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanRDepositorInsertTaTasR
TagsTwinStrepII-bdSUMOExpressionBacterialAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMtb ATR
Plasmid#138297PurposeExpression of Mtb ATRDepositorInsertCorrinoid adenosyl transferase
TagsHis tagExpressionBacterialPromoterT7Available SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-CjCas9
Plasmid#89754PurposeExpression of CjCas9 with His tag in E.coliDepositorInsertCjCas9
UseCRISPRTags6XHis, HA, and SV40 NLSExpressionBacterialPromoterT7Available SinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only