Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 28,500 results for: REP

Showing: 8981 - 9000 of 28500 results

  2. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid
    #101218
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A and H698A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101218-plasmid-map-sequence-id-198035

    Enlarge

  5. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

    Plasmid
    #217676
    Purpose
    AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.
    Depositor
    Joseph Raimondo
    Article
    Selfe et al bioRxiv 2023.08.30.555464
    Insert
    GtACR2-P2A-Voltron2ST
    Use
    AAV
    Tags
    Soma targeting sequence (Kv2.1) on Voltron2 gene …
    Expression
    Mammalian
    Mutation
    Promoter
    EF‐1α
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217676-plasmid-map-sequence-id-432468

    Enlarge

  6. LSB-hsa-miR-199a-3p

    Plasmid
    #103319
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-199a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-199a-3p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103319-plasmid-map-sequence-id-195687

    Enlarge

  7. pCAGMKOSiE

    Plasmid
    #20865
    Depositor
    Keisuke Kaji
    Article
    Kaji et al Nature. 2009 Mar 1. ():.
    Insert
    myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    20865-plasmid-map-sequence-id-9737

    Enlarge

  10. pFRLuc-CoV2-delStem1

    Plasmid
    #177616
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    3-nucleotide (UAC) deletion in Stem 1
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177616-plasmid-map-sequence-id-350042

    Enlarge

  17. pIgG1-2.1

    Plasmid
    #225831
    Purpose
    Mouse IgG1 chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG- and Strep2-tagged monoclonal antibodies for use as tag-specific ELISA standards.
    Depositor
    Gabriel Victora
    Article
    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:
    Insert
    Mouse anti-CGG, IgG1 heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225831-plasmid-map-sequence-id-447625

    Enlarge

  18. pFBP_2xNbLYS1::LUZ

    Plasmid
    #189918
    Purpose
    Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.
    Depositor
    Adam Steinbrenner
    Article
    Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Various
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    189918-plasmid-map-sequence-id-377067

    Enlarge

Showing: 8981 - 9000 of 28500 results