We narrowed to 28,500 results for: REP

1. pLenti PA-NL

   Plasmid

   #113453

   Purpose

   Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-NanoLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti PA-mCit

   Plasmid

   #113451

   Purpose

   Lentiviral ProteinA-mCitrine expression vector

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBbC

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2 Notch1 LNG (CC→SS)-6MT

   Plasmid

   #41739

   Depositor

   Raphael Kopan

   Article

   Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

   Insert

   Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 leader peptide (aa 1-23) …

   Promoter

   Available since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TetO-FUW-Hes3

   Plasmid

   #61535

   Purpose

   Expresses Hes3 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Hes3 (Hes3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

   Plasmid

   #101235

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant F491A/M495A/T496A/N497A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   F491A, M495A, T496A, N497A and Q926A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

   Plasmid

   #101234

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant T657A/G658A/W659A/R661A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   T657A, G658A, W659A, R661A and Q926A

   Promoter

   T7

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

   Plasmid

   #101220

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A and Q926A

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

   Plasmid

   #101232

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G528A, V583A, E584A, D585A, N588A and Q926A

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FHpHG_mTent5c_LxxAA (L3m)

   Plasmid

   #221441

   Purpose

   Transfection into the cells for the reporter or qPCR assay.

   Depositor

   Marcos Morgan

   Article

   Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

   Insert

   Tent5c_LXXAA (L3m) (Fam46c Mouse)
   

   Use

   Tags

   FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

   Expression

   Mammalian

   Mutation

   L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

   Promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG391

    Plasmid

    #226732

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas3-APOBEC1

    Plasmid

    #229536

    Purpose

    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.26-Ccnd2PromoterRegion1(1.2kb)

    Plasmid

    #228058

    Purpose

    1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Ccnd2 promoter (Ccnd2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.26-Ccnd2PromoterRegion2(0.5kb)

    Plasmid

    #228059

    Purpose

    0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Ccnd2 promoter (Ccnd2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.26-Igf2Promoter

    Plasmid

    #228060

    Purpose

    0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Igf2 promoter (Igf2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG517

    Plasmid

    #226735

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG43

    Plasmid

    #226736

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAN056

    Plasmid

    #220052

    Purpose

    Reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    fLuc-CFTR (exons 22-27) (CFTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAN057

    Plasmid

    #220053

    Purpose

    NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.3846G>A mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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