We narrowed to 20,480 results for: Cre

Showing: 9081 - 9100 of 20480 results

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1 pegRNA1 with trimmed attP

Plasmid

#222346

Purpose

pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA1

Use

CRISPR

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh4

Plasmid

#168988

Purpose

Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRi(v1)-Blast

Plasmid

#170067

Purpose

Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMTL5

Plasmid

#127967

Purpose

DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 1

Plasmid

#193600

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 1 (AP2M1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LUC

Plasmid

#218630

Purpose

This plasmid contains luciferase under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LUC (LOC116160065 )

Use

Lentiviral, Luciferase, and Synthetic Biology

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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VPX-mRNA

Plasmid

#227679

Purpose

plasmid for VPX mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

VPX (HIV2gp3 )

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2

Plasmid

#75112

Purpose

lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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WFS1-Flag-pcDNA3

Plasmid

#13011

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-STAT3

Plasmid

#111934

Purpose

Expresses human STAT3 fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Signal transducer and activator of transcription 3 (STAT3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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CROP-seq-opti

Plasmid

#106280

Purpose

A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-iT7

Plasmid

#211699

Purpose

NIS-Seq-compatible lentiviral vector for expressing sgRNAs

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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wtBxb1

Plasmid

#222337

Purpose

Plasmid for expressing wild type Bxb1 in mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wild type Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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