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PEP109E

Plasmid

#97395

Purpose

binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAC90-pmax-DEST

Plasmid

#48222

Purpose

Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR and Gateway Cloning

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJMC-L1-Donor-Esp3I

Plasmid

#197842

Purpose

Donor vector to restore L1 destination via Esp3I

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS253

Plasmid

#114271

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Mutation

*please see depositor comment below

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2-wPRE-pA

Plasmid

#60489

Purpose

eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

Depositor

Boris Fehse

Article

Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

Insert

IRES-eGFP; wPRE; BGH-pA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2-EM7neoPheS

Plasmid

#112923

Purpose

piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

neoPheS

Use

Piggybac transposon gateway destination vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mCherry-C1-WPRE

Plasmid

#159956

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mCherry-C1

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only
pLKO.1 RagD shRNA #2

Plasmid

#30322

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
NCD-GFP

Plasmid

#24507

Depositor

Ron Vale

Article

Case et al Cell. 1997 Sep 5. 90(5):959-66.

Insert

NCD (ncd Fly)

Tags

GFP and His

Expression

Bacterial

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pOGG037

Plasmid

#113995

Purpose

SC module. Super-folder GFP green fluorescence reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Super-folder GFP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-YPET-C1-WPRE

Plasmid

#159954

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

YPET-C1

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pB6-Gfap-T2A-tdTomato-FNF-DTA

Plasmid

#140271

Purpose

B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Gfap (Gfap Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tdTomato

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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SAFB1-pcDNA3.1

Plasmid

#32742

Depositor

Steffi Oesterreich

Article

Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

Insert

SAFB1 (SAFB Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pSBDEST.B

Plasmid

#79460

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #1

Plasmid

#26627

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pDL1

Plasmid

#182263

Purpose

Gibson cloning vector for synthesis of single and double stranded RNA

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Type

Empty backbone

Use

Gibson cloning vector for synthesis of single and…

Promoter

T3 and SP6 for sense and antisense riboprobe synt…

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRK Smad3 deltaC Flag

Plasmid

#12626

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad3 (aa 1-382)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

Plasmid

#225924

Purpose

Expressing LGC-47 in AVA neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

rig-3

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBAV150

Plasmid

#79764

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Jean Greenberg

Article

Vinatzer et al Mol Microbiol. 2006 Oct;62(1):26-44. Epu

Type

Empty backbone

Use

Tetr

Expression

Plant

Promoter

DEX

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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