We narrowed to 59,461 results for: %s

1. pFX-Tom20-Venus

   Plasmid

   #174187

   Purpose

   Express Venus with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with mitchondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of TOM20

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacPAK8-His-myc-Uba1

   Plasmid

   #29508

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

   Insert

   Uba1 (UBA1 Budding Yeast)
   

   Tags

   His and myc

   Expression

   Insect

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

   Plasmid

   #13571

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta11 Aphrodite
   

   Tags

   Aphrodite and Ares

   Expression

   Bacterial

   Mutation

   Fluorophore-derived linker shortened. F16A mutati…

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUHD Cdk2 DN HA

   Plasmid

   #27651

   Depositor

   Greg Enders

   Article

   Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

   Insert

   Cdk2 (CDK2 Human)
   

   Tags

   HA (YDVPDYA)

   Expression

   Mammalian

   Mutation

   D146N (Dominant Negative)

   Available Since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-ABE

   Plasmid

   #107723

   Purpose

   For expression of ABE7.10 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Insert

   ABE7.10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

   Plasmid

   #69224

   Purpose

   Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS and Zif268

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-ecDHFR-G51PEKN

   Plasmid

   #118582

   Purpose

   Bacterial expression of E.coli DHFR G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Expression

   Bacterial

   Mutation

   G51 mutated to P51; Glu-Lys-Asn residues inserted…

   Promoter

   T7

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSV HA eIF2beta WT

   Plasmid

   #45898

   Depositor

   Louise Larose

   Article

   Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

   Insert

   Eif2s2 (Eif2s2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pmCellFree _KA2_ZIKV NS5

   Plasmid

   #145368

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS5
   

   Tags

   8xHis and mCherry

   Expression

   Bacterial

   Promoter

   T7- CMV/CAG

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mPA-GFP-Paxillin-22

    Plasmid

    #57153

    Purpose

    Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Article

    Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

    Insert

    Paxillin
    

    Tags

    mPA-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. Human SOX17 sgRNA

    Plasmid

    #59725

    Purpose

    Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hSox17 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pPK1E-2+t

    Plasmid

    #48148

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL-RbUTR

    Plasmid

    #20883

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    Rb 3'UTR (Rb1 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pET22b-ecDHFR-Y100F

    Plasmid

    #110826

    Purpose

    Bacterial expression of E.coli DHFR Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    Y100 mutated to F100

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA_tdtomato_reporter_1_ST1_2x_binding

    Plasmid

    #79368

    Purpose

    Assays activity of transcriptional activators fused to ST1 Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-myc-CD4-Sema4D

    Plasmid

    #51603

    Purpose

    expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    CD4-Sema4D
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Sema4D amino acids 1-754 have been replaced with …

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Human BLIMP1 sgRNA

    Plasmid

    #59724

    Purpose

    Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hBLIMP1 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Tol2-lyzC-microRNA 199-3-Dendra2

    Plasmid

    #97119

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    microRNA 199 3 Dendra2
    

    Use

    Zebrafish

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

    Plasmid

    #141052

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSN24

    Plasmid

    #100109

    Purpose

    expresses a chimeric SOX2-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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