161,805 results

1. GCaMP-AMPK-SPARK

   Plasmid

   #226257

   Purpose

   Dual reporter of Ca2+ levels and AMPK activity

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   GCaMP-AMPK-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFA6a URA3

   Plasmid

   #61924

   Purpose

   Expression of S. cerevisiae URA3 cloned into pFA6a

   Depositor

   Jon Houseley, David Tollervey

   Article

   Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

   Insert

   URA3
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MultiMate-Cell-cycle

   Plasmid

   #206280

   Purpose

   Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
   

   Use

   Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEmerald-Alpha-Actinin2-N-18

   Plasmid

   #53988

   Depositor

   Michael Davidson

   Insert

   Alpha-Actinin2
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pCAIP-hXRCC1wt-eGFP

   Plasmid

   #206032

   Purpose

   Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   hXRCC1
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.tgoGAP1

   Plasmid

   #78737

   Purpose

   Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   tgoGAP1
   

   Use

   Tags

   N terminal 81 amino acids of human galactosyltran…

   Expression

   Mammalian

   Mutation

   D117A/D119A/D163A in aequorin moiety

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. p5E-mmCdh5pro (JDW 1239)

   Plasmid

   #224478

   Purpose

   Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Cdh5 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB511

   Plasmid

   #74853

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-GFAP-iGABASnFR2-WPRE

   Plasmid

   #218871

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   GFAP

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

    Plasmid

    #216478

    Purpose

    lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AcGFP-MSH2

    Plasmid

    #215121

    Purpose

    Expresses AcGFP-MSH2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH2 (MSH2 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2155

    Plasmid

    #141948

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL11A (BCL11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. nifF in pET21b

    Plasmid

    #124520

    Purpose

    Azotobacter vinelandii flavodoxin II of Nitrogenase

    Depositor

    Douglas Rees

    Article

    Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

    Insert

    Flavodoxin II (nifF Azotobacter vinelandii)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7 / lac

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 10 x UFR-luc

    Plasmid

    #153158

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX317 GFP

    Plasmid

    #193681

    Purpose

    Constitutive lentiviral expression of GFP

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDisplay-NE2m-IRES-mCherry-CAAX

    Plasmid

    #208690

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-EGR1

    Plasmid

    #192913

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3290

    Plasmid

    #144766

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CTCF (CTCF Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

    Plasmid

    #216479

    Purpose

    lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    wtYAP (YAP1) (YAP1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge