We narrowed to 22,063 results for: his

1. pDONR P4-P1R-V5-6xHis

   Plasmid

   #48346

   Purpose

   Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   V5 and 6xHis epitope tags cassette
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Contains a Kozak sequence and initiation codon up…

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc-His-zfCsf1a

   Plasmid

   #168103

   Purpose

   Express soluble His-tagged zebrafish Csf1a in insect cells

   Depositor

   Petr Bartunek

   Article

   Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

   Insert

   Csf1a (csf1a Zebrafish)
   

   Use

   Tags

   6x His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. APCS-bio-His

   Plasmid

   #53424

   Purpose

   Expresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   APCS (APCS Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB-­NIC-­II-­HisN-­TET12

   Plasmid

   #45496

   Depositor

   Roman Jerala

   Article

   Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

   Insert

   TET12
   

   Use

   Synthetic Biology

   Tags

   6xHistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-ARF1-V5-His6

   Plasmid

   #67432

   Purpose

   Mammalian expression of hArf1 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAS1-his-24::tagRFP

   Plasmid

   #124329

   Purpose

   Expressing Nuclear Localized tagRFP

   Depositor

   Andrew Leifer

   Insert

   tagRFP (his-24 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sema3a(L, del3)-AP-His

   Plasmid

   #72010

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-­His-­zfTpo

   Plasmid

   #64309

   Purpose

   Express soluble His-tagged zebrafish thrombopoietin in insect cells

   Depositor

   Petr Bartunek

   Article

   Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

   Insert

   Thrombopoietin (thpo Zebrafish)
   

   Use

   Tags

   (His)6

   Expression

   Insect

   Mutation

   Thrombopoietin starts at AA37 (AA1-36 encode the …

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

9. His6-SMT3-SopB

   Plasmid

   #183676

   Purpose

   Recombinant Salmonella Typhimurium SopB

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA-MNase-CaHIS1

    Plasmid

    #162462

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7-His-GFP-Arp11

    Plasmid

    #51402

    Purpose

    expression of GFP-tagged mouse Arp11 under T7 promoter

    Depositor

    Trina Schroer

    Article

    Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

    Insert

    Arp11 (Actr10 Mouse)
    

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. His-ZZ-TEV-BICDR1

    Plasmid

    #111859

    Purpose

    Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Use

    Tags

    His-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST26-his-hSFRP1

    Plasmid

    #189539

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2-hFAM 3B-His

    Plasmid

    #172192

    Purpose

    Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

    Depositor

    Edward De Robertis

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    hFAM 3B
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTricHis-ILY-prepore

    Plasmid

    #160519

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTricHis-ILY-WT-Y336W

    Plasmid

    #160515

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-WT-Y336W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y336W

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS426-TUB1-internal6xHis

    Plasmid

    #60390

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4)

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. WT-short-UBASH3A-V5-6xHis

    Plasmid

    #192102

    Purpose

    Mammalian expression of human wild-type short-form UBASH3A with V5-6xHis tag

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 6xHis-WT-long-UBASH3A

    Plasmid

    #192091

    Purpose

    Mammalian expression of human wild-type long-form UBASH3A with 6xHis tag

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b-His-IkappaBalpha(FL)

    Plasmid

    #44738

    Depositor

    Gourisankar Ghosh

    Insert

    IkappaBalpga (Nfkbia Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pKT-SCFP3A-HIS

    Plasmid

    #64686

    Purpose

    pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    SCFP3A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SERA5-bio-His

    Plasmid

    #50817

    Purpose

    Expresses enzymatically monobiotinylated full length SERA5

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA5
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGF/HisB-cGK1

    Plasmid

    #106451

    Purpose

    expression of murine cyclic GMP-dependent kinase

    Depositor

    Georg Weber

    Article

    He et al Biochem Biophys Res Commun. 2004 Aug 13;

    Insert

    cyclic GMP-dependent kinase (Prkg1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CALCR-bio-His

    Plasmid

    #53412

    Purpose

    Expresses full-length Calcitonin receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CALCR (CALCR Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFA6a-RGS18HIS-kanMX6

    Plasmid

    #26882

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    18xHis tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RAMA-bio-His

    Plasmid

    #50737

    Purpose

    Expresses enzymatically monobiotinylated full length RAMA ectodomain

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RAMA
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TMED1-bio-His

    Plasmid

    #51832

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED1 (TMED1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28_6xHis-PARP1-A762V

    Plasmid

    #174795

    Purpose

    Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A762V (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A762V

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge