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p178 Grg-1L Myc His

Plasmid

#11067

Depositor

Ramesh Shivdasani

Publication

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-L (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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1479 pcDNA3 flag HA 14-3-3 gamma

Plasmid

#9000

Depositor

William Sellers

Insert

14-3-3 gamma (Ywhag Rat)

Tags

HA and flag

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLT61

Plasmid

#1690

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, unc224616, unc2218148, unc2218082, unc223827…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti_PE7-2A-BSD

Plasmid

#234073

Purpose

For lentiviral expression of PE7 with blasticidin resistance

Depositor

Greg Findlay

Publication

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PE7

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Publication

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple Gaq

Plasmid

#196058

Purpose

Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphaQ-RLuc8 (GNAQ Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Bxb1-LP-v2-TC

Plasmid

#194326

Purpose

AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_iRFP670 sgBFP

Plasmid

#192478

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A iRFP670

Expression

Mammalian

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_lox

Plasmid

#183762

Purpose

Bxb1-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Publication

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Publication

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P1TDPGH023

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Expression

Mammalian

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-hSyn1-GFP

Plasmid

#177810

Purpose

Encodes a GFP driven by human synapsin I promoter

Depositor

Christopher Grigsby

Publication

Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

Insert

CopGFP inserted behind human synapsin I promoter (SYN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Syn1

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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mito-meGFP

Plasmid

#172481

Purpose

meGFP targeted to the mitochondrial matrix with a human COX8 presequence

Depositor

Thomas Schwarz

Insert

Mito

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PEA1-GFP

Plasmid

#171993

Purpose

Delivers all prime editing nickase components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Publication

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC28-MacroGreen

Plasmid

#160665

Purpose

Expresses GFP fusion of a multisite mutant of the Af1521 protein

Depositor

Herwig Schüler

Publication

Garcia-Saura et al Commun Biol. 2021 Jul 28;4(1):919. doi:

Insert

Af1521 modified synthetic construct eGFP fusion

Tags

eGFP and hexahistidine_TEV recognition

Expression

Bacterial

Promoter

LacI

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Publication

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Publication

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Mutation

contains an altered Cre gene modified by the addi…

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-Gαi*-BERKY3

Plasmid

#158198

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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