We narrowed to 9,669 results for: control

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pAAV-VTKD4-ChR2-YFP

Plasmid

#178721

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 1 CMV mAG h-Geminin

Plasmid

#206281

Purpose

ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mAG-hGeminin (GMNN Human)

Use

Multimate/gateway entr 1

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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TRCN0000072225

Plasmid

#78160

Purpose

shLacZ control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

LacZ

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pMMK ENTR 1 CMV mAG beta-catenin

Plasmid

#206258

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mAG Ctnnb1 (Ctnnb1 Mouse)

Use

Multimate/gateway entr 1

Tags

monomeric Azami Green

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Fgf4enhLV (FpG12)

Plasmid

#69444

Purpose

positive control plasmid for lentiviral reporter expression in ESCs

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Inserts

minimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
FGF4 enhancer (Fgf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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MEK1-pcw107-V5

Plasmid

#64650

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cas9-T2A-STOP-TdT

Plasmid

#126425

Purpose

Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Mutation

alanine-6 of TdT has been changed to a STOP codon

Promoter

pCMV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTUU

Plasmid

#159749

Purpose

Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

Depositor

Wolfgang Lukowitz

Article

Angulo et al bioRxiv

Insert

gRNA scaffold

Use

CRISPR

Expression

Plant

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65453

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV1

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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TRCN0000072254

Plasmid

#78161

Purpose

Luciferase control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Luciferase

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFD3-Act5c

Plasmid

#130278

Purpose

Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

pCFD3-Act5c (Act5C Fly)

Use

CRISPR

Expression

Insect

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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TRCN0000231725

Plasmid

#78163

Purpose

shRFP control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

RFP

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
Lyn-FLINC-EKAR1(TA)

Plasmid

#87708

Purpose

Negative-control mutant of Lyn-FLINC-EKAR1.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Lyn-FLINC-EKAR1(TA)

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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TRCN0000231737

Plasmid

#78162

Purpose

Luciferase control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Luciferase

Use

RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pEMS1097

Plasmid

#29028

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-ChR2-YFP

Plasmid

#178725

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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TRCN0000072213

Plasmid

#78159

Purpose

shRFP control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

RFP

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only