We narrowed to 11,803 results for: nsf

1. pAAV-CaMKIIa-hChR2(E123A)-EYFP

   Plasmid

   #35505

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-VTKD2

   Plasmid

   #170847

   Purpose

   AAV backbone for Cre-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 SV40-CMV-SaCas9-3xNLS

   Plasmid

   #78601

   Purpose

   AAV vector containing SaCas9

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   SV40-CMV-SaCas9-3xNLS
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   SV40, CMV promoters

   Available Since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

4. ROSA26(MultiFPsΔPuro)

   Plasmid

   #140759

   Purpose

   Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Inserts

   puromycin-N-acetyltransferase
   Venus
   mCherry
   mCerulean
   

   Use

   Mouse Targeting

   Promoter

   CAGGS

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

   Plasmid

   #132685

   Purpose

   AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Carnosine dipeptidase 2 (Cndp2 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   Chicken b-actin

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-ASAP2s

   Plasmid

   #101276

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn (human synapsin)

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

   Plasmid

   #100855

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   jRGECO1b
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSyn1.Twitch2B.WPRE.SV40

   Plasmid

   #100040

   Purpose

   AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Has Service

   AAV1

   Insert

   Twitch2B
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

   Plasmid

   #170175

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-ST-P2A-NLS-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2284

    Plasmid

    #158419

    Purpose

    AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    AAV

    Promoter

    Ple349

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-DIO-tTA

    Plasmid

    #127091

    Purpose

    An AAV genome with Cre-dependent expression of tTA from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CAG

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-Aion-citrine

    Plasmid

    #192569

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

    Plasmid

    #98930

    Purpose

    AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    iGluSnFr
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-gfaABC1D-HA-deLACCO1-NGR

    Plasmid

    #208099

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    AAV

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Mutation

    D10A;H840A nuclease-deficient

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-HA-eLACCO2.1-NGR

    Plasmid

    #207989

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell, Yuki Kambe

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    AAV

    Promoter

    CaMKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TfR-moxGFP-DHFR

    Plasmid

    #173012

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-moxGFP-DHFR
    

    Tags

    DHFR and moxGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_FKBP-V5-TWIST1

    Plasmid

    #215022

    Purpose

    AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Use

    AAV

    Tags

    FKBP-V5

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CamKII-ArchT-GFP (PV2527)

    Plasmid

    #99039

    Purpose

    AAV-mediated expression of ArchT under the CamKII promoter

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    ArchT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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