We narrowed to 10,053 results for: ADA

1. cr3Vn

   Plasmid

   #184861

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.TBG mCherry-LDTS-eLIR

   Plasmid

   #189002

   Purpose

   lipophagy-inducing adaptor protein, AAV

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   LDTS-eLIR, mCherry
   

   Use

   AAV

   Tags

   HA

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTJV1Sc3-rpoB

   Plasmid

   #166983

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   RecT (recT E. coli)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pVvanCC

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKB01_tagRFP

   Plasmid

   #69103

   Purpose

   Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   tagRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8r-R153del

   Plasmid

   #208296

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-R153del-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS406-PHO5-GFP-hFAPP1 PH domain

   Plasmid

   #58723

   Purpose

   Expresses GFP-Tagged human FAPP1 PH domain in yeast

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
   

   Tags

   GFP and Myc

   Expression

   Yeast

   Promoter

   PHO5

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV.TBG mCherry-LDTS-LIRmut

   Plasmid

   #189003

   Purpose

   lipophagy-inducing adaptor protein, AAV, negative control

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   LDTS-LIRmut, mCherry
   

   Use

   AAV

   Tags

   HA

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. cr3Ec

   Plasmid

   #184860

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnxABE-flgn

   Plasmid

   #187932

   Purpose

   A-to-G base editor

   Depositor

   Yi Xiao

   Article

   et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

   Insert

   ecTadA(8e)-nxCas9 (D10A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEINO4

    Plasmid

    #179488

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    aadA1
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKB01_mOrange2

    Plasmid

    #69102

    Purpose

    Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mOrange2
    

    Use

    Synthetic Biology

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

    Plasmid

    #202719

    Purpose

    Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Myr-Pink Flamindo
    EGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX3x-hRanBP2-(IR1+M)

    Plasmid

    #53140

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2631-2711)

    Promoter

    tac

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. RanBP2-RB3-4-pET23a

    Plasmid

    #85769

    Purpose

    bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2304-3062)

    Promoter

    T7

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE:CloverCP-EFS:ZeoR

    Plasmid

    #68478

    Purpose

    Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    CloverCP
    Zeocin Resistance
    

    Use

    Lentiviral

    Tags

    "CP" degradation domain

    Expression

    Mammalian

    Promoter

    EFS and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

    Plasmid

    #163062

    Purpose

    Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    QuasAr2
    

    Use

    Gateway entry vector

    Tags

    mOrange2

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRTP801/TA-ODD

    Plasmid

    #206212

    Purpose

    An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    tetracycline-responsive transactivator fused with oxygen-dependent degradation domain
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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