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Plasmid#216860PurposeCIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-Malat1 gRNA
UseLentiviralTagsExpressionMammalianMutationPromoterSyn1, U6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL01009::AtU6p
Plasmid#46968PurposeEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.DepositorInsertAtU6p
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT2T3
Plasmid#50591PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, U6-29t plus, U6-1p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT3T4
Plasmid#50592PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-1tplus, U6-26p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Circular guide cloning vector
Plasmid#180184PurposeCloning vector for expressing circular RNA from a U6 promoterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0040-LwaCas13a crRNA backbone
Plasmid#103851PurposeBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAADepositorInsertU6-LwaCas13aDR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-yA
Plasmid#191015PurposeCRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.DepositorInsertyA
UseCRISPRTagsExpressionMutationPromoterAspergillus flavus U6Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
NmCas9_sgRNA_expression_in_pBluescript
Plasmid#122091PurposeU6 driven NmCas9 sgRNA expression vector for cloning own guidesDepositorInsertNmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL90003
Plasmid#68262PurposeLevel 0 Golden Gate Part: Promoter U6 (Triticum aestivum)DepositorInsertPromoter: U6 (Triticum aestivum)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiUniversal-Puro
Plasmid#127749PurposeLentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL90002
Plasmid#68261PurposeLevel 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)DepositorInsertPromoter: U6-26 (Arabidopsis thaliana)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAGFP
Plasmid#58255PurposeU6 promoter-driven single guide RNA complementary to eGFPDepositorInsertU6.gRNAGFP cassette
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM38869
Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAS1
Plasmid#58252PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locusDepositorInsertU6.gRNAS1 cassette
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir144 EF1Alpha-puro-T2A-BFP
Plasmid#164791PurposeExpress miR-144 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only