2,226 results

1. pDC146

   Plasmid

   #169366

   Purpose

   Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

   Depositor

   Axel Brunger

   Article

   Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45

   Insert

   Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
   

   Use

   Tags

   6 his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV5-rat ERK2-D319N

   Plasmid

   #40820

   Depositor

   Natalie Ahn

   Article

   Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

   Insert

   MAPK1 (Mapk1 Rat)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   D319N

   Promoter

   CMV

   Available since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. mEos3.2-Homer1-C-18

   Plasmid

   #57460

   Purpose

   Localization: Membrane Adaptor, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   Homer1 (Homer1 Rat)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. FUp95bGW (B6)

   Plasmid

   #74015

   Purpose

   lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion

   Depositor

   Weifeng Xu, Oliver Schluter, Robert Malenka

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   Psd95 beta isoform
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant Psd95

   Promoter

   hUbC

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-parkin C431S

   Plasmid

   #45976

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin C431S (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PS-CFP2-PSD95-N-14

   Plasmid

   #57246

   Purpose

   Localization: Nucleus, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   PSD95
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pMM2-rPMCA3b

   Plasmid

   #47763

   Purpose

   mammalian expression of rat PMCA3b

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

   Insert

   PMCA3b (Atp2b3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

8. Jag2ICD-HA pBacPAK8-3

   Plasmid

   #17332

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Jagged2 (Jag2 Rat)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

9. rEgr3/LZRS

   Plasmid

   #27785

   Depositor

   David Wiest

   Insert

   Egr3 (Egr3 Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

10. prLCA-Citrine

    Plasmid

    #40271

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    Clta (Clta Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Syt2

    Plasmid

    #186688

    Purpose

    Expresses Synaptotagmin 2 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    Synaptotagmin 2
    

    Use

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1 AT1AR-324delta

    Plasmid

    #45635

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-324delta (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    added stop codon at 324 aa

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pHR'-ROMK1-CITE-GFP

    Plasmid

    #21322

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

    Insert

    ROMK1 (Kcnj1 Rat)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pSG5-HA-CaMKKbeta rat FL WT

    Plasmid

    #33321

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S3V, G96R, and P108L

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-4T-1-3xMyc-ERK2(K52R)

    Plasmid

    #129228

    Purpose

    Encodes Myc tagged human ERK2(K52R) for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    ERK2(K52R) (Mapk1 Rat)
    

    Use

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    Changed Lysine 52 to Arginine

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

    Plasmid

    #179073

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Scarlet(rfp)-Link-2xPH
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179059

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRFP shRNA-resistant TrkB WT

    Plasmid

    #72714

    Purpose

    Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

    Depositor

    Freddy Jeanneteau

    Article

    Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

    Insert

    TrkB
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

    Promoter

    CMV

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pXL-CAG-LAP2-NLG1

    Plasmid

    #43924

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    LAP-NLG1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 Glt1b

    Plasmid

    #98706

    Purpose

    mammalian expression of Glt1b

    Depositor

    Paul Rosenberg

    Article

    Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

    Insert

    Glt1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I348V and N365Y (please see depositor comments se…

    Promoter

    CMV

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFlg167

    Plasmid

    #101019

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    cmv

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. G protein alpha-s-mCerulean

    Plasmid

    #66084

    Purpose

    G protein alpha-s internally tagged with mCerulean and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCR3-FLAG-Gephyrin E

    Plasmid

    #68819

    Purpose

    Flag tagged mammalian expression of E domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    internal EcoRI site has silent mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

24. mTurquoise-Lysosomes-20

    Plasmid

    #55568

    Purpose

    Localization: Lysosome Membrane, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. SEPT11 sh1 shRNA

    Plasmid

    #122020

    Purpose

    Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-HA-DNM2-W525L

    Plasmid

    #155263

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tryptophan 525 to Leucine

    Promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pORANGE Tubb3-4xGFP KI

    Plasmid

    #183445

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 4xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Myc-delta

    Plasmid

    #118037

    Purpose

    GABAA receptor expression (delta subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRD (Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge