We narrowed to 22,189 results for: ESC

1. CeNL(Ca2+)_19μ-pcDNA3

   Plasmid

   #111927

   Purpose

   Cyan color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_19μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E67D, F92W, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMB-TGN61r

   Plasmid

   #61175

   Purpose

   Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtSYP61
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBW-m4046854

   Plasmid

   #145759

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 leader protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pJB045 (ZapB-mEos2)

   Plasmid

   #72651

   Purpose

   Inducible expression of ZapB-mEos2 in bacteria

   Depositor

   Jie Xiao

   Article

   Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

   Insert

   ZapB-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4046832

   Plasmid

   #145691

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pZA-mCherry-R5

   Plasmid

   #106258

   Purpose

   Expresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetO

   Depositor

   Urartu Seker

   Article

   Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

   Insert

   mCherry
   

   Use

   Tags

   Added silaffin R5 peptide to C terminus of mCherr…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

   Plasmid

   #133340

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Nano-lantern(Ca2+)_117/pcDNA3

   Plasmid

   #51985

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. dCas12j3 KRAB

   Plasmid

   #188507

   Purpose

   Expresses FLAG tagged dCas12j3 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046953

    Plasmid

    #145764

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

    Plasmid

    #159898

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTorPE-FRISZ

    Plasmid

    #176888

    Purpose

    Far-red fluorescent zinc indicator FRISZ in pTorPE vector

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    FRISZ
    

    Use

    Tags

    Periplasmic expression TorA tag and Twin-Strep-ta…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046890

    Plasmid

    #145740

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pZyxin-mMaple2

    Plasmid

    #101150

    Purpose

    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    human codon optimized mMaple2 fused to zyxin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046883

    Plasmid

    #145693

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Nano-lantern(Ca2+)_600/pRSETB

    Plasmid

    #51976

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pGBW-m4046841

    Plasmid

    #145578

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Nano-lantern-Actin/pEGFP-C1

    Plasmid

    #51972

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. P199

    Plasmid

    #139717

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, Luz promoter is circadian controlled

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_D_pAtELF4:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtELF4

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Nano-lantern-peroxisome/pcDNA3

    Plasmid

    #51974

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    SKL

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

22. Nano-lantern(Ca2+)_300/pRSETB

    Plasmid

    #51977

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

23. CT-Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51990

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis and chloroplast transit peptid

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pACYC-Skp-hLYZ

    Plasmid

    #83924

    Purpose

    Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    lysozyme (LYZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGBW-m4046876

    Plasmid

    #145669

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pGBW-m4046726

    Plasmid

    #145756

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGBW-m4046945

    Plasmid

    #145668

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pGBW-m4046947

    Plasmid

    #145705

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGBW-m4046804

    Plasmid

    #145595

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pGBW-m4046766

    Plasmid

    #145612

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pCMB-TMEg

    Plasmid

    #61209

    Purpose

    Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-GFP-BCP
    

    Use

    Tags

    EGFP

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. P315-FBP_10

    Plasmid

    #139701

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Nano-lantern(Ca2+)_11/pRSETB

    Plasmid

    #51981

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-4GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pMB1-spect-PthrC3-eGFP

    Plasmid

    #107409

    Purpose

    This plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC3

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CT-Nano-lantern(ATP1)/pENTR1A

    Plasmid

    #51991

    Purpose

    Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Entry vector

    Tags

    chloroplast transit peptid

    Expression

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pGBW-m4046926

    Plasmid

    #145663

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51988

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid

    #133344

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_cpaA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pTEI128

    Plasmid

    #126209

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGBW-m4046964

    Plasmid

    #145681

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. SEPLuc

    Plasmid

    #141102

    Purpose

    bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGBW-m4046950

    Plasmid

    #149258

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. psecBFP2

    Plasmid

    #62230

    Purpose

    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

    Depositor

    Erik Snapp

    Article

    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

    Insert

    secBFP2
    

    Use

    Tags

    secbfp2

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pGBW-m4046971

    Plasmid

    #145664

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pGBW-m4046755

    Plasmid

    #145573

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pGBW-m4046905

    Plasmid

    #145762

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pGBW-m4046844

    Plasmid

    #145590

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pGBW-m4046834

    Plasmid

    #149247

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. pGBW-m4046908

    Plasmid

    #145603

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge