We narrowed to 25,261 results for: nes

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_CLIP_CAP_Gly

Plasmid

#178470

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLIP_CAP_Gly (CLIP1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_MDM2_SWIB

Plasmid

#178481

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

MDM2_SWIB (MDM2 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNA4_Arm

Plasmid

#178478

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-Cameleon

Plasmid

#123351

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

Plasmid

#171587

Purpose

Bacterial expression of NLS-RfxCas13d-NLS 6His

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

MKIEE-NLS-RfxCas13d-NLS-HA-6His

Tags

HA-6HIS and MKIEE

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PhLP1-myc-6xHis

Plasmid

#218176

Purpose

Expresses phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKD072

Plasmid

#136472

Purpose

SSB-C-term-GFP fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-C-term-GFP (ssb E. coli)

Tags

superfolder GFP

Expression

Bacterial

Mutation

superfolder GFP attached to C-terminus at Phe 178

Promoter

T7 promoter

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2-S127A

Plasmid

#17794

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pETM33_MAD2L1_HORMA

Plasmid

#178480

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

MAD2L1_HORMA (MAD2L1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_TNKS_TANK-peptide-bind

Plasmid

#178489

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

TNKS_TANK-peptide-bind (TNKS Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike-D614G

Plasmid

#166850

Purpose

For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

Depositor

Neville Sanjana

Article

Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

Insert

SARS-CoV-2 Spike (S SARS-CoV-2 virus)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47870

Purpose

All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
OCT4 crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ΔN-RNMT

Plasmid

#112708

Purpose

Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG and HIV Rev nuclear export signal (NES)

Expression

Mammalian

Mutation

deleted amino acids 2-120, mutated active-site D2…

Promoter

CMV

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pETM33_KPNA4_Arm

Plasmid

#178495

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-Cameleon

Plasmid

#123352

Purpose

Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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