We narrowed to 1,790 results for: pGEX

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pGEX-4T1-Cdc42 G15A

Plasmid

#69356

Purpose

expression of nucleotide free Cdc42 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-parkin 141-465

Plasmid

#45971

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 141-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T2 Parkin RBR

Plasmid

#31255

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Parkin (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

RBR domain, residues 217-465

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-EZH2-T487A mutant

Plasmid

#28061

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Mutation

T487A

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-4T Syt1 C2AB(140) WT

Plasmid

#134695

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-GST-YAP-S127A

Plasmid

#33053

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

GST

Expression

Mammalian

Mutation

S127A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 NLS-GFP

Plasmid

#129441

Purpose

bacterial expression of GFP

Depositor

Oskar Laur

Insert

GFP

Expression

Bacterial

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX C_K nsp1 CoV2

Plasmid

#175516

Purpose

Expresses C_K for bioconjugation on nsp1

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

C_K nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Tags

SNAP

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-C / 2523 JM15

Plasmid

#13311

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-422 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-4T2 SHP2 E76A

Plasmid

#8324

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

E76A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pGEX6P1-Ubl-sr1(SIRPT1)

Plasmid

#75298

Purpose

Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 1-339

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3-1-182

Plasmid

#22115

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 183-373 deleted

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-parkin 219-465

Plasmid

#45972

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 219-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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