159,589 results

1. LentiV_Cas9_puro

   Plasmid

   #108100

   Purpose

   Lentiviral expression plasmid of spCas9 with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-hPar2

   Plasmid

   #53228

   Purpose

   mammalian expression of human Par2

   Depositor

   Shaun Coughlin

   Article

   Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

   Insert

   Par2 (F2RL1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1 N-term GFP tTA

   Plasmid

   #107551

   Purpose

   All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP (optional)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET his-eGFP

   Plasmid

   #113551

   Purpose

   For bacterial expression and purification of eGFP

   Depositor

   Jeanne Stachowiak

   Article

   Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

   Insert

   his-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A206K

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLKO-mcherry-luc-puro-TRPM1 cDNA

   Plasmid

   #29782

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   TRPM1 (TRPM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-HP1alpha

   Plasmid

   #179703

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2 RFP670

   Plasmid

   #187646

   Purpose

   lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

   Depositor

   Martin Carroll

   Insert

   RFP 670
   

   Use

   Lentiviral

   Tags

   RFP670 and sgRNA cloning site

   Expression

   Mutation

   Promoter

   EFS (P2A)

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158653

    Purpose

    making transgenics expressing a UAS driven mCherry and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Tags

    Expression

    Mutation

    F70A/F108Y

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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