We narrowed to 3,430 results for: Agt

1. CAG-mCherry-Otx2shRNA1

   Plasmid

   #73979

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPR_TRAC

   Plasmid

   #164993

   Purpose

   Expression of gRNA targeting TCRalpha constant locus

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against TRAC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

4. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pOC2 Dlg4-Halo-FKBP KI

   Plasmid

   #183435

   Purpose

   CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo-FKBP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TET-pLKO.1 PURO shGFP

   Plasmid

   #83085

   Purpose

   Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

7. CROP_C9_Puro_PDGFRA

   Plasmid

   #183313

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-H1-SGIPZ_NF1 sh1miR

   Plasmid

   #119206

   Purpose

   knockdown of NF1

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   sh1miR targeting NF1 (NF1 Rat, Human, Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB9337 (pgRNA_IV-1_NatMX)

   Plasmid

   #161590

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOC2 Dlg4-Halo KI

    Plasmid

    #183449

    Purpose

    CreOFF knock-in for PSD95-Halo (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

13. PX458_NFIL3_2

    Plasmid

    #86349

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX458_NFIL3_1

    Plasmid

    #86348

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSU6-shTim23/CMV-eGFP

    Plasmid

    #89795

    Purpose

    Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

    Depositor

    Robert Friedlander

    Article

    Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

    Insert

    shRNA Tim23 (Timm23 Mouse)
    

    Use

    Tags

    eGFP under separate promoter

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOC4 Actb-mRuby3 KI

    Plasmid

    #183437

    Purpose

    FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and mRuby3 donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR9.924

    Plasmid

    #39539

    Depositor

    Katharina Strub

    Article

    Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

    Insert

    SRP9 (SRP9 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. CROP_C9_Puro_FGFR2

    Plasmid

    #183290

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets FGFR2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    FGFR2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRRNA-G0-R0

    Plasmid

    #101803

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CAG-EGFP-Otx2shRNA2

    Plasmid

    #73982

    Purpose

    Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    EGFP-Otx2-shRNA2
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pILGFP9E3

    Plasmid

    #185842

    Purpose

    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CAG-EGFP-Otx2shRNA1

    Plasmid

    #73981

    Purpose

    Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLY134

    Plasmid

    #130962

    Purpose

    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. plentiCRISPR-sgMDH1

    Plasmid

    #72875

    Purpose

    The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgMDH1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pADH118-2

    Plasmid

    #90984

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PX458_ZGPAT_2

    Plasmid

    #86328

    Purpose

    Encodes gRNA for 3' target of human ZGPAT

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ZGPAT (ZGPAT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge