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Showing: 2351 - 2400 of 13557 results

  10. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Christoph Bock
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219680-plasmid-map-sequence-id-462884

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  40. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  41. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  42. pBD21_035

    Plasmid
    #109152
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    EGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV and pTet
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109152-plasmid-map-sequence-id-209236

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Showing: 2351 - 2400 of 13557 results