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pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A) C716A

Plasmid

#24605

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, C716A

Promoter

Available since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735

Plasmid

#60633

Purpose

ZKSCAN1 circular RNA expression plasmid containing minimal sufficient introns

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Sense

Plasmid

#60638

Purpose

Expresses the human EPHB4 exon 11/12 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

David S. Weiss, Craig Ellermeier

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJH3577

Plasmid

#179612

Purpose

Pceh-12::Chrimson::Cherry unc-54 3' UTR C.elegans VB-MNs expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Use

Tags

Cherry

Expression

Worm

Mutation

Promoter

Pceh-12

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-DAAT-V33G/T242G

Plasmid

#159278

Purpose

Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

Depositor

Roberto Chica

Article

Parmeggiani et al ACS Catalysis 9, 3482–3486.

Insert

Engineered d-alanine aminotransferase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Valine 33 and threonine 242 have been mutated to …

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJH3558

Plasmid

#179611

Purpose

Punc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Use

Tags

Cherry

Expression

Worm

Mutation

Promoter

Punc-4

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Antisense

Plasmid

#60640

Purpose

Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Stephen Lory, Cammie Lesser

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pUC119-Padh81-GBP-mCherry(N)-hphMX6-lys1*

Plasmid

#89072

Purpose

genomic GBP-mCherry epitope N-terminal tagging at lys1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJK429

Plasmid

#155376

Purpose

PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT

Plasmid

#24606

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, Y459A

Promoter

Available since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) C716A

Plasmid

#24607

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, Y459A, C716A

Promoter

Available since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCS2 YFP-Smo-Weyer's

Plasmid

#41014

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Use

Tags

EVC2 (aa 1177-1220) and YFP

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Promoter

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV Lex VP16 (F442A) HA (P#1710)

Plasmid

#14595

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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