We narrowed to 28,411 results for: REP

1. pLV-Enh eGFP Reporter-muKC2-X1

   Plasmid

   #59200

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt1-ex1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

   Plasmid

   #207031

   Purpose

   Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-mCherry-NES reporter (pDN160)

   Plasmid

   #72660

   Purpose

   NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Insert

   NLS-mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28-Strep-OPA1(MGD) (SB264)

   Plasmid

   #227613

   Purpose

   Inducible expression of Strep-tagged Opa1(MGD) in E.coli

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Tags

   Twin-StrepTag

   Expression

   Bacterial

   Mutation

   contains aa 253-580 (MGD) and aa 938-960

   Promoter

   T7

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB1-A11(2-196, G38R)-Strep

   Plasmid

   #196209

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G38R

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-AU1

   Plasmid

   #162113

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-HA-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-HA-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-FLAG

   Plasmid

   #162092

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-HA-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-HA-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HBV 1.3-mer P-null replicon

   Plasmid

   #65462

   Purpose

   containing 1.3 units of the P-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

   Insert

   1.3 units of the P-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

    Plasmid

    #113620

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7a sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

11. T7 promoter + filler DNA reporter plasmid

    Plasmid

    #156454

    Purpose

    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    NeoR/KanR
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7 and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid

    #177328

    Purpose

    Control vector for Flp-dependent DREADD constructs

    Depositor

    Ulrik Gether

    Insert

    mCherry
    

    Use

    AAV; Flp-frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-6xGLY-Strep-tagII-HIS3MX6

    Plasmid

    #20756

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid

    #197872

    Purpose

    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Inserts

    part kilA/repL
    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains only the 24 bp of 3'-end of kilA ge…

    Promoter

    Bba_J23115 and PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG001 His6-TwinStrep-SUMO-AapCas12b

    Plasmid

    #153162

    Purpose

    Bacterial expression plasmid for AapCas12b

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

    Insert

    AapCas12b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFlag-tdMCP-Erk2 (pErk reporter)

    Plasmid

    #226167

    Purpose

    Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-Erk2 (MAPK1 Human)
    

    Use

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid

    #157933

    Purpose

    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

    Insert

    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    

    Use

    Tags

    sfGFP & TwinStrep

    Expression

    Mammalian

    Mutation

    E190D

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-TWIN-STREP-FLAG-NPRL2

    Plasmid

    #164985

    Purpose

    NPRL2 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    NPRL2 (NPRL2 Human)
    

    Use

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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