We narrowed to 15,384 results for: pET

Showing: 9721 - 9740 of 15384 results

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LentiCRISPRCreb5gRNA

Plasmid

#195021

Purpose

Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Creb5 gRNA (CREB5 Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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p4949 pLPCX-His-Xpress NLS hBrd4 CTD

Plasmid

#14459

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Use

Retroviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMG058 MBP-GS 634-666-His

Plasmid

#154077

Purpose

Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GYS1 (GYS1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Cav2.2 D122A HA pCAGGS

Plasmid

#206097

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV/B-actin

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZDeltaAL

Plasmid

#99551

Purpose

Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Mutation

replaced 1557-1618 with SGGSG

Promoter

T7

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTsc1#1/Cre

Plasmid

#173657

Purpose

Expresses a Tsc1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tsc1 (Tsc1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTsc1#2/Cre

Plasmid

#173658

Purpose

Expresses a Tsc1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tsc1 (Tsc1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#2/Cre

Plasmid

#173648

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#1/Cre

Plasmid

#173647

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mqgDUX4mqg*

Plasmid

#21177

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

Point mutation at nucleotide 6163 (numbering acco…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
Lenti-sgNotch2#2/Cre

Plasmid

#193226

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch2#2 (Notch2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PE VQR

Plasmid

#162797

Purpose

All-in-one prime editor piggyBac transposon, VQR variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-BRCA1 1364-1437

Plasmid

#71119

Purpose

Mammalian expression of GFP-BRCA1 1364-1437

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1364-1437 of BRCA1

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-sgNcor1#2/Cre

Plasmid

#173606

Purpose

Expresses a Ncor1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ncor1 (Ncor1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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DSPQ-HTT-TALEN2 BSD

Plasmid

#92246

Purpose

TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CAG

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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DSPQ-HTT-TALEN1 ZEO

Plasmid

#92245

Purpose

TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CAG

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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