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mNeonGreen-LIG3

Plasmid

#215143

Purpose

Expresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

LIG3 (LIG3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

P438

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

Plasmid

#110560

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

stRBS-lacZ

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7repeats)-GFP-Pho8

Plasmid

#207011

Purpose

Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

GFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA_MET_D14_3Flag

Plasmid

#182495

Purpose

Contains cDNA of the MET gene without the exon 14

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

MET (MET Human)

Tags

C-terminal 3xFlag tag

Expression

Mammalian

Mutation

Exon 14 deleted

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_hCOL4A4-3FLAG

Plasmid

#198101

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A4 (COL4A4 Human)

Use

Lentiviral

Tags

3FLAG

Promoter

EF1a

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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Active Wnt7B-V5

Plasmid

#43817

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT7B (WNT7B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pKD184

Plasmid

#90953

Purpose

Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23114, PltetO-1, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_Pkinase

Plasmid

#109933

Purpose

Protein expression and purification of PLK1_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_Pkinase

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-LMNB1-IRES2-mCherry

Plasmid

#159599

Purpose

Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-LMNB1-IRES2-mCherry

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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EF.STAT3C.Ubc.GFP

Plasmid

#24983

Purpose

3rd generation lentiviral expression of constitutively active Stat3

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

A662C, N664C (constitutively active)

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P-R

Plasmid

#189849

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pH-A3A-PBE

Plasmid

#119774

Purpose

Conversion of C to T in rice plants transformation

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2FA CO Tol2 TPase

Plasmid

#133032

Purpose

Expression vector for production of codon optimized Tol2 transposase

Depositor

Charles Nathan Hancock

Article

Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

Insert

Codon optimized Tol2 Transposase

Use

In vitro expression

Mutation

Codon Optimized

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKD182

Plasmid

#90952

Purpose

Expresses ThsS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Ptac

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_mEGFP-SNAP-tag2

Plasmid

#226513

Purpose

Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

mEGFP-SNAP-tag2

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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