164,577 results

1. pUGI-NLS

   Plasmid

   #101091

   Purpose

   Expresses UGI in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

   Insert

   UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST/N1-hBIN1-GFP

   Plasmid

   #27305

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPAmCherry1-C1

   Plasmid

   #31929

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNitET-SacB-kan

   Plasmid

   #107692

   Purpose

   Expression of the phage Che9c RecET system for Recombineering in mycobacteria

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Inserts

   Che9 phage RecET
   PNit2-NitR
   SacRB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Synapsin-pm-iATPSnFR1.0

   Plasmid

   #102555

   Purpose

   expresses membrane displayed ATP sensor in neurons

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204189

   Purpose

   Doxycycline inducible lentiviral vector for human GATA6 overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   GATA6 (GATA6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-Cebpa

   Plasmid

   #139834

   Purpose

   doxycycline-inducible expression of mouse Cebpa in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Cebpa (Cebpa Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ASAP2f

   Plasmid

   #83960

   Purpose

   Fluorescent reporter for voltage imaging

   Depositor

   Michael Lin

   Article

   Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

   Insert

   ASAP2f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGolden-AAV

    Plasmid

    #51424

    Purpose

    Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    lacZ
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-NES-ZapCV2

    Plasmid

    #222950

    Purpose

    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2 (NES Synthetic)
    

    Use

    Tags

    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-eTLR

    Plasmid

    #214737

    Purpose

    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    AAVS1-eTLR (PPP1R12C Synthetic, Bovine, CMV, Anguilla japonica, influenza, Human, Chicken, Rabbit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMax_mPlum

    Plasmid

    #177830

    Purpose

    Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWM_12x601_45bpLinker

    Plasmid

    #157791

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_45bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBCW

    Plasmid

    #62658

    Purpose

    Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL-FLKif3A

    Plasmid

    #13742

    Depositor

    Linda Wordeman

    Article

    Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

    Insert

    mouse Kif3A (Kif3a Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. ePE6d

    Plasmid

    #229029

    Purpose

    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

    Depositor

    Li Juan

    Article

    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-PD-L1-SNAP

    Plasmid

    #223577

    Purpose

    Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORANGE LOX Cloning template vector

    Plasmid

    #139651

    Purpose

    Cloning template for ORANGE-CAKE conditional knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ERG_pet28a

    Plasmid

    #131642

    Purpose

    Bacterial expression of ETS gene ERG

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ERG (ERG Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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