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We narrowed to 2,167 results for: CO

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Showing: 1921 - 1960 of 2167 results

  1. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  3. qTAG-N-Puro-mStayGold-ACTB

    Plasmid
    #227320
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227320-plasmid-map-sequence-id-452091

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  6. pETDuet-TFIIF

    Plasmid
    #171082
    Purpose
    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171082-plasmid-map-sequence-id-335551

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  8. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available Since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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  14. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  15. pQ-NFY-A-GB-HC

    Plasmid
    #171081
    Purpose
    Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    NF-YA (NFYA Human)
    NF-YB (NFYB Human)
    NF-YC (NFYC Human)
    Tags
    GST and His
    Expression
    Bacterial
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171081-plasmid-map-sequence-id-335631

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  17. TFORF0511

    Plasmid
    #143037
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143037-plasmid-map-sequence-id-399005

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  19. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

    Plasmid
    #217676
    Purpose
    AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.
    Depositor
    Joseph Raimondo
    Article
    Selfe et al Nat Commun. 2024 Oct 16;15(1):8913. doi:
    Insert
    GtACR2-P2A-Voltron2ST
    Use
    AAV
    Tags
    Soma targeting sequence (Kv2.1) on Voltron2 gene …
    Expression
    Mammalian
    Promoter
    EF‐1α
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217676-plasmid-map-sequence-id-432468

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  20. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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  21. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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  32. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

    Plasmid
    #122982
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-BoNT/B(1-146)-iLID
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    iLID contains long lived V416I mutation
    Promoter
    human synapsin
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122982-plasmid-map-sequence-id-238428

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  40. TFORF2386

    Plasmid
    #143589
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MBD2 (MBD2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    143589-plasmid-map-sequence-id-391946

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Showing: 1921 - 1960 of 2167 results