We narrowed to 34,402 results for: CaS;
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Plasmid#188503PurposeExpresses FLAG tagged LbdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD832APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV261-Cas12a
Plasmid#158707Purposeexpresses codon-optimized FnCpf1DepositorInsertFnCpf1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FOG1[N+C]
Plasmid#100085PurposedCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertFOG1(aa 1-45) (ZFPM1 Human)
UseCRISPRTags3XFLag-NLS-FOG1-dCas9-FOG1-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCasperAUG-Gal4-X
Plasmid#8378DepositorInsertYeast Gal4 (GAL4 fused to first ~30 amino acids of Drosophila ADH, Budding Yeast)
UseDrosophila p-element vectorTagsExpressionMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9pUbi-N
Plasmid#106331PurposeExpression of Cas9 in plants, ZmUbi promoter, Kanamycin selectionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…PromoterZmUbiAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-RZ-Cas12j2
Plasmid#189784PurposeGolden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT1
Plasmid#210549PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
UseTagsExpressionPlantMutation2bp insertion after AAVS1 target sequence that di…PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
UseTags6X His and sortase A sorting motifExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 TadDE
Plasmid#193846PurposeExpress TadDE (with SaCas9) in mammalian cellsDepositorInsertTadDE with SaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-dCas9-PBE
Plasmid#98162PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseRiceTagsExpressionPlantMutationPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionTagsExpressionMutationRice codon optimizedPromoter2x35SAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux
Plasmid#176239PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.PAC
Plasmid#58329PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p1195-pssAAV.U1a.hNme2Cas9
Plasmid#129534PurposeAAV vector expressing Nme2Cas9DepositorInserthuman codon-optimized Nme2Cas9
UseAAVTags2xNLS and NLS-3xHA-NLSExpressionMutationPromoterU1aAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9
Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR
Plasmid#81251PurposeLentiviral construct for building stable cell lines expressing Cas9 via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only