We narrowed to 1,209 results for: proc.2

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pRRG36-ChAT

Plasmid

#99069

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ChAT (choline acetyltransferase) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG199-bmp4

Plasmid

#99076

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Bmp4 (also known as bmp) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Djun - antisense

Plasmid

#38338

Depositor

Norbert Perrimon

Publication

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Expression

Insect

Promoter

Hsp70

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
Myc-PSFΔRRM1+2

Plasmid

#35267

Depositor

Benjamin Blencowe

Publication

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-429

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pSuper mouse DGCR8-2

Plasmid

#14773

Depositor

Tyler Jacks

Publication

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pBI-CMV1-mir144-CMV2-mir1

Plasmid

#164634

Purpose

Express miR-144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

mir144 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Publication

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Tags

mNeonGreen

Expression

Bacterial

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRRG43-sFRP1

Plasmid

#99071

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

sFRP1 (secreted frizzled related protein 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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BiP 2: C. elegans BiP (hsp-4)-GFP

Plasmid

#21896

Depositor

David Ron

Publication

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

BiP (hsp-4 Nematode)

Tags

GFP

Expression

Worm

Mutation

Fusion between the GFP and hsp-4 takes place at A…

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

Plasmid

#175050

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Publication

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(V266E)

Plasmid

#90089

Purpose

Expresses caspase 3 V266E mutant in bacterial cells

Depositor

Clay Clark

Publication

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

V266E

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Publication

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11

Plasmid

#172068

Purpose

Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Publication

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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psicheck-2-Mut

Plasmid

#48164

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Publication

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Mut

Use

Luciferase

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Sfxn3-mod

Plasmid

#48170

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

Depositor

Vamsi Mootha

Publication

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of SFXN3 (SFXN3 Human)

Use

Luciferase

Mutation

A/T mutation in the ATG of the uORF

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

Plasmid

#138310

Purpose

Lenti expression of AcrIIA13b

Depositor

Jennifer Doudna

Publication

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13b

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-hCOL1A1

Plasmid

#140111

Purpose

Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

Depositor

Sergey Leikin

Insert

Type I procollagen α1 (COL1A1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

COL1A1 exons 2-3 replaced by fluorescent tag

Promoter

CMV

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRRG272-eya

Plasmid

#99085

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

eyes absent (eya)

Use

T/a cloning vector for dsrna generation and the g…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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