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pSP64T3 BMP2 pro dnmVg1 (DM#182)

Plasmid

#15074

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

Insert

BMP2-Vg1 dom neg

Use

Sp6 expression for use in xenopus

Tags

Expression

Mutation

dominant negative version of BMP2-Vg1 chimera. Th…

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3B2 G120A

Plasmid

#123076

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-CTSL

Plasmid

#172414

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Procathepsin L (Cathepsin L1) (CTSL Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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ASAP2f

Plasmid

#83960

Purpose

Fluorescent reporter for voltage imaging

Depositor

Michael Lin

Article

Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

Insert

ASAP2f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Rab6

Plasmid

#31734

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab6 (Rab6 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pGL4.11_Nued2_E2

Plasmid

#203831

Purpose

Luciferase reporter plasmid for Igf1 enhancer 2

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shCrh2

Plasmid

#132710

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(2) (Crh Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(V266E)

Plasmid

#90089

Purpose

Expresses caspase 3 V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

V266E

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 G120

Plasmid

#123077

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 no-stop

Plasmid

#123207

Purpose

Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-TAPBPR-TM

Plasmid

#178645

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha

Plasmid

#136434

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Use

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Mutation

Promoter

Dox inducible Tet-ON promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5

Plasmid

#31733

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab5 (Rab5 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pET21b-Caspase-3(D3A,V266E)

Plasmid

#90091

Purpose

Expresses caspase D3A and V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A V266E

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

Plasmid

#195853

Purpose

Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

Depositor

Andrew Leifer

Article

Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

Insert

Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)

Use

Tags

Expression

Worm

Mutation

Promoter

lim-4

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB2

Plasmid

#200982

Purpose

Mammalian expression plasmid for myc-tagged EphB2

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB2 (EPHB2 Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-GRM3-VN

Plasmid

#98965

Purpose

FLAG-tagged human mGluR3 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Use

Tags

FLAG (dykddddk) epitope tag, Signal/leader sequen…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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