163,381 results

1. pEGFP-Atg14L

   Plasmid

   #21635

   Depositor

   Tamotsu Yoshimori

   Article

   Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

   Insert

   Atg14L (ATG14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF CAG luc-EGFP-cre puro

   Plasmid

   #67503

   Purpose

   Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   luciferase-EGFP-cre
   

   Use

   Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVFLAG-ZFP423

   Plasmid

   #24764

   Depositor

   Bruce Spiegelman

   Article

   Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

   Insert

   Zinc Finger Protein 423 (Zfp423 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2010

   Availability

   Academic Institutions and Nonprofits only

4. HA-KIF5B*-strep

   Plasmid

   #120170

   Purpose

   Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   HA and Streptavidin

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD4_mCherry

   Plasmid

   #187386

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSelAct-KO

   Plasmid

   #174374

   Purpose

   Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

   Depositor

   Gitta Coaker

   Article

   Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

   Type

   Empty backbone

   Use

   Vector for 2-step knockout by homologous recombin…

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-FOXO3a WT

   Plasmid

   #1787

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. modRNAc0-Cas9-2A-Puro

   Plasmid

   #172855

   Purpose

   IVT template plasmid for making modified Cas9Puro RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9-2A-Puro
   

   Use

   Ivt template plasmid

   Tags

   2A-Puro

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2861

   Plasmid

   #143644

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SREBF2 (SREBF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN1-PinkyCaMP

    Plasmid

    #232861

    Purpose

    A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMpGE010

    Plasmid

    #71536

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4_10xUAS-MLPmin-luc2

    Plasmid

    #194383

    Purpose

    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    10xUAS-luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MLP minimal

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. wt dynamin 2 pEGFP

    Plasmid

    #34686

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406G, V664A, H692R and Q758R

    Promoter

    CMV

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJPT002-M-HaloTag-1x FLAG

    Plasmid

    #202493

    Purpose

    Express "M" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Myristoylation site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVITRO1-dV-IgE/λ

    Plasmid

    #61879

    Purpose

    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMV306hsp

    Plasmid

    #26155

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST53-LRRK2-R1441C

    Plasmid

    #25046

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Arginine 1441 mutated to Cysteine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pET26b_AT118-L-V5-His

    Plasmid

    #222848

    Purpose

    E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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