19,830 results

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pRsetB-pHRed

Plasmid

#31472

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Tags

7xHis

Expression

Bacterial

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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GeNL/pcDNA3

Plasmid

#85200

Purpose

Green color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Green enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mTurquoise2-CNA35

Plasmid

#61602

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mTurqouise and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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YFC43

Plasmid

#89488

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP

Depositor

Alejandro Ferrando

Article

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-C (aa 154-240)

Expression

Plant

Promoter

2x35S

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-HNRNPA1C-mCh-Cry2WT

Plasmid

#101226

Purpose

hnRNPA1(186-320) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

hnRNPA1(186-320) (HNRNPA1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM8031

Plasmid

#48037

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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PGC-1 alpha promoter luciferase delta CRE

Plasmid

#8888

Depositor

Bruce Spiegelman

Article

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter dCRE (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Site-directed mutagenesis to remove the CREB bind…

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

Plasmid

#64613

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Tags

V5

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-p14

Plasmid

#42330

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p14 (LAMTOR2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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GPRC6A-Tango

Plasmid

#66386

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPRC6A (GPRC6A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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CXCR4-Tango

Plasmid

#66262

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CXCR4 (CXCR4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pVITRO1-102.1F10-IgE/λ

Plasmid

#50365

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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dxCas(3.7)-VPR

Plasmid

#108383

Purpose

Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.7)-VPR

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-IC2-FL

Plasmid

#51409

Purpose

expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-FLuc

Plasmid

#170575

Purpose

Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

Luc

Use

Luciferase and Retroviral

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGST-PSP-hTG

Plasmid

#85470

Purpose

bacterial expression of human tissue transglutaminase

Depositor

Jeffrey Keillor, Ray Truant

Article

Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

Insert

hTG2 (TGM2 Human)

Tags

GST and PSP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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