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RIP-Timer (DM#285)

Plasmid

#15109

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

insulin II promoter (Ins2 Rat)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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Lamp1-RFP

Plasmid

#1817

Purpose

Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

lysosome associated membrane protein 1 (Lamp1 Rat)

Tags

RFP

Expression

Mammalian

Mutation

D50E (not important for function of plasmid)

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pSEP-LAMP1-RFP

Plasmid

#167970

Purpose

Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

Depositor

Juan Bonifacino

Article

Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

LAMP1 (Lamp1 Rat)

Tags

SEP and mRFP

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7s

Plasmid

#104463

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7s

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Article

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Expression

Mammalian

Promoter

Wnt promoter

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVTREGCaMP6f

Plasmid

#97410

Purpose

Amplified expression of GCaMP6f in combination with TET driver

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

TRE3

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CMV mScarlet-LC3B

Plasmid

#200431

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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CMV mRuby3-Synapsin1a

Plasmid

#187896

Purpose

For labeling pre-synaptic terminals

Depositor

Timothy Ryan

Insert

Synapsin1a

Tags

mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-Lysosomes-20

Plasmid

#55902

Purpose

Localization: Lysosome Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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SNAP-OMP25

Plasmid

#141149

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat, Budding Yeast)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti6-AMPK alpha1 (1-312)

Plasmid

#162131

Purpose

Expression vector for constitutively active AMPK alpha 1

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

Insert

AMPK alpha1 (1-312) (PRKAA1 Rat)

Use

Lentiviral

Tags

V5

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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SnFR-gamma2

Plasmid

#165495

Purpose

Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

Depositor

Andrew Plested

Article

Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-RSV-jGCaMP7s-W

Plasmid

#126690

Purpose

Mammalian calcium sensor protein.

Depositor

Darrell Kotton

Insert

jGCaMP7s

Use

Lentiviral

Tags

6xHIS, T7, Xpress

Mutation

A52V, A317L

Promoter

RSV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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