2,326 results

1. pCI pHluorin Syt12

   Plasmid

   #184513

   Purpose

   Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 12 (Syt12 Rat)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs GFP-LC3-RFP

   Plasmid

   #117413

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-H2BmGreenLantern

   Plasmid

   #177332

   Purpose

   To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

   Depositor

   Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B (Hist1h2bq Rat)
   

   Use

   AAV

   Tags

   mGreenLantern

   Expression

   Mammalian

   Mutation

   This fusion protein was optimized to the Human co…

   Promoter

   CAG

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1A-C1A-EYFP

   Plasmid

   #61155

   Purpose

   Diacylglycerol (DAG) reporter

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C1A from PKCG amino acids 26-89

   Promoter

   CMV

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLAMP1-emiRFP670

   Plasmid

   #136570

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LAMP1-emiRFP670
   

   Tags

   emiRFP670

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF Synaptophysin-mRuby

   Plasmid

   #188980

   Purpose

   Synaptic buton marker

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Synthetic, Rat)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-14-3-3 gamma

   Plasmid

   #13274

   Depositor

   Michael Yaffe

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK7-HA-S6K1-WT

    Plasmid

    #8984

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKT-CNP-inact

    Plasmid

    #211804

    Purpose

    Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Expression

    Bacterial

    Mutation

    H73L H153L

    Promoter

    tet

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKT-CNP

    Plasmid

    #211803

    Purpose

    Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Expression

    Bacterial

    Promoter

    tet

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-OMP25

    Plasmid

    #141150

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS426GFP-2×PH(PLCδ)

    Plasmid

    #36092

    Depositor

    Scott Emr

    Article

    Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

    Insert

    2xPH(PLCdelta) fusion
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    contains aa11-140 of PLCδ (including the PH domai…

    Promoter

    PRC1

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-GLUT4-7myc-GFP

    Plasmid

    #52872

    Purpose

    detection and measurement of GLUT4 translocation

    Depositor

    Jonathan Bogan

    Article

    Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

    Insert

    GLUT4 (Slc2a4 with human c-terminus, Rat)
    

    Use

    Retroviral

    Tags

    7x-myc (internal) and GFP

    Expression

    Mammalian

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-CMV-NES-jRCaMP1b

    Plasmid

    #63136

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1b
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LAMP1-mRFP-FLAG

    Plasmid

    #34611

    Purpose

    3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

    Depositor

    David Sabatini

    Article

    Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    2xFlag and mRFP

    Expression

    Mammalian

    Available Since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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