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GFP-C1(2)delta

Plasmid

#21216

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-N2-PKCdelta-C1 (Prkcd Rat)

Tags

GFP

Expression

Mammalian

Mutation

C1(2) domains of PKC delta (rat) (Lab plasmid WC…

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pEYFP-NR1a

Plasmid

#17928

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)

Tags

EYFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO2

Plasmid

#125244

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Expression

Mammalian

Mutation

W44Y, D432A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mScarlet-LAMP1

Plasmid

#185138

Purpose

Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

Depositor

Andrew York

Article

Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

Insert

Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)

Tags

mScarlet

Expression

Mammalian

Mutation

deleted amino acids 1-21 of LAMP1

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_NR1.

Plasmid

#170457

Purpose

Expresses histidine tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

Hexa-histidin

Expression

Mammalian

Promoter

Synapsin

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 K44A

Plasmid

#128153

Purpose

Expression of Dynamin 2 K44A

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Mutation

K44A

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria1-GFP KI

Plasmid

#131489

Purpose

Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Article

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFR3.v857.GPI

Plasmid

#178331

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.GPI

Plasmid

#178335

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 Wild Type

Plasmid

#128152

Purpose

Expression of Dynamin 2

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.PDGFR

Plasmid

#178333

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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