2,226 results

1. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV.hSyn.iGluSnFR3.v857.SGZ

   Plasmid

   #178330

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGP-CMV-NES-jRCaMP1b

   Plasmid

   #63136

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1b
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mRuby2-Lysosomes-20

   Plasmid

   #55902

   Purpose

   Localization: Lysosome Membrane, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   LAMP1
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pAAV.CAG.iGluSnFR3.v857.GPI

   Plasmid

   #178335

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. beta-arrestin2 mYFP

   Plasmid

   #36917

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   beta-arrestin2 (Arrb2 Rat)
   

   Use

   Tags

   mYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. TGN38 EGFP

   Plasmid

   #128148

   Purpose

   Expression of TGN38 amino acids 304-357

   Depositor

   Jennifer Lippincott-Schwartz

   Insert

   TGN38 (Tgoln2 Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Contains amino acids 304-357

   Promoter

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBK-YEE-BE3

   Plasmid

   #85177

   Purpose

   Expresses YEE-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   YEE-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-CMV-jGCaMP7f

   Plasmid

   #104483

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7f
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-Lysosomes-20

    Plasmid

    #55073

    Purpose

    Localization: Lysosome Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Use

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mEmerald-Cx43-7

    Plasmid

    #54055

    Purpose

    Localization: Gap Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

    Insert

    Cx43
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3_mScarlet-LAMP1

    Plasmid

    #185138

    Purpose

    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

    Depositor

    Andrew York

    Article

    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

    Insert

    Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-21 of LAMP1

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP2u2-mCherry

    Plasmid

    #27672

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Ap2m1 (Ap2m1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.GFAP.iGluSnFR3.v857.SGZ

    Plasmid

    #178337

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. K44A HA-dynamin 2 pcDNA3.1

    Plasmid

    #34685

    Depositor

    Sandra Schmid

    Insert

    dynamin 2ba
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LAMP1-mRFP-FLAG

    Plasmid

    #34611

    Purpose

    3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

    Depositor

    David Sabatini

    Article

    Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    2xFlag and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB-GLUT4-7myc-GFP

    Plasmid

    #52872

    Purpose

    detection and measurement of GLUT4 translocation

    Depositor

    Jonathan Bogan

    Article

    Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

    Insert

    GLUT4 (Slc2a4 Rat, with human c-terminus)
    

    Use

    Retroviral

    Tags

    7x-myc (internal) and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge