We narrowed to 629 results for: CA2

1. pGEX-2T-IQGAP1

   Plasmid

   #30107

   Depositor

   David Sacks

   Article

   Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET30b-7Y-SrtA

   Plasmid

   #105601

   Purpose

   expresses 7Y sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7Y mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAD-D4

   Plasmid

   #37473

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D4 second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ara

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pBAD-D2

   Plasmid

   #37470

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D2 second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ara

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDN3.1(+)-CaNARi

   Plasmid

   #64848

   Purpose

   FRET-based calcineurin (CaN) activation biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET30b-7D-SrtA

   Plasmid

   #105600

   Purpose

   expresses 7D sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7D mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-D2cpV

   Plasmid

   #37471

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D2cpv second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pTH1-SaSrtA-Q

   Plasmid

   #64980

   Purpose

   Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108Q

   Promoter

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Mouse NCKX6 (pcDNA3.1+)

   Plasmid

   #75215

   Purpose

   Mammalian expression of SLC8B1

   Depositor

   Jonathan Lytton

   Article

   Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

   Insert

   SLC8B1 (Slc8b1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcpmAmetrine

    Plasmid

    #42170

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    cpmAmetrine173
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pTH1-SaSrtA-A

    Plasmid

    #64979

    Purpose

    Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108A

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTH1-SaSrtA-K

    Plasmid

    #64978

    Purpose

    Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lyn-R-GECO1

    Plasmid

    #120410

    Purpose

    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

    Depositor

    Won Do Heo

    Article

    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

    Insert

    Lyn-R-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TPC1-G-GECO1.2

    Plasmid

    #207143

    Purpose

    Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPCN1 (TPCN1 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Myc-IQGAP1

    Plasmid

    #30118

    Depositor

    David Sacks

    Article

    Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-4mtD3cpv

    Plasmid

    #36324

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
    

    Use

    Tags

    First 29 aa of human COX subunit 8a

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-D1ER

    Plasmid

    #36325

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1ER second generation cameleon (calcium sensor) targeted to ER
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Use

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-OMM-jRCaMP1b

    Plasmid

    #127871

    Purpose

    Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-PM-CaNAR2

    Plasmid

    #64730

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal targeting sequence from Lyn…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-tag2B-PISP/AIPP1

    Plasmid

    #47606

    Depositor

    Emanuel Strehler

    Article

    Goellner et al Ann N Y Acad Sci. 2003 Apr;986:461-71.

    Insert

    PISP (PDZD11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA-D1cpV

    Plasmid

    #37479

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1cpV second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-lynD3cpV

    Plasmid

    #37472

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D3cpv second generation cameleon (calcium sensor)
    

    Use

    Tags

    the myristoylation and palmitoyla- tion sequence …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

28. CaYin1 NES

    Plasmid

    #33318

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    CaYin1 NES
    

    Use

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+)-PM-CaNARi

    Plasmid

    #64850

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNARi
    

    Use

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-D1

    Plasmid

    #37480

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1 second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

31. CaYin1

    Plasmid

    #33067

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    CaYin1
    

    Use

    Tags

    6 His tag

    Expression

    Bacterial

    Mutation

    Promoter

    ARA promoter

    Available since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTH1-SaSrtA-KA

    Plasmid

    #64981

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Stim1-CaRQ

    Plasmid

    #34560

    Depositor

    Kevin Truong

    Article

    Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

    Insert

    Stim1-CaRQ (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Q63L in RhoA

    Promoter

    CMV, T7

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EGFP-hPMCA4b

    Plasmid

    #47589

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA4x/b (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K442R and P673L relative to NP_001675.3

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TPC2-G-GECO1.2

    Plasmid

    #207141

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-ER-CaNAR2

    Plasmid

    #64732

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 27 amino acids from CYP450,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-ER-AKAR4

    Plasmid

    #64733

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    AKAR4
    

    Use

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Lentiviral CaMKKbeta

    Plasmid

    #33322

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    human PGK

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. rNrx1beta(47-299)

    Plasmid

    #20173

    Depositor

    Axel Brunger

    Article

    Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

    Insert

    Neurexin 1 beta (Nrxn1 Rat)
    

    Use

    Tags

    6X His tag

    Expression

    Insect

    Mutation

    no splice site

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

44. Lentiviral FLAG-CaMKKbeta

    Plasmid

    #32458

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

45. rNL1(46-638)

    Plasmid

    #20172

    Depositor

    Axel Brunger

    Article

    Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

    Insert

    Neuroligin 1 (Nlgn1 Rat)
    

    Use

    Tags

    C-terminal 6X His tag

    Expression

    Insect

    Mutation

    no splice sites

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

46. pcDNA3.1(+)-ER-CaNARi

    Plasmid

    #64852

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNARi
    

    Use

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. FRB-CFP-ras

    Plasmid

    #87451

    Purpose

    rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    CFP and ras fragment

    Expression

    Mammalian

    Mutation

    FKBP and rapamycin binding domain only

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. Rat SERCA3 (pMT2)

    Plasmid

    #75185

    Purpose

    Mammalian expression of ATP2A3

    Depositor

    Jonathan Lytton, Gary Shull

    Article

    Burk et al J Biol Chem. 1989 Nov 5;264(31):18561-8.

    Insert

    ATP2A3 (Atp2a3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. CaM-RhoA(DP)-Venus

    Plasmid

    #31994

    Depositor

    Kevin Truong

    Article

    Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

    Insert

    CaM-RhoA(DP) (RHOA Human)
    

    Use

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Q63L mutation in RhoA, E7S and D65Y in CaM

    Promoter

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

50. pLyn-CaRQ

    Plasmid

    #34559

    Depositor

    Kevin Truong

    Article

    Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

    Insert

    pLyn-CaRQ (RHOA Human)
    

    Use

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Q63L in RhoA

    Promoter

    CMV, T7

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only