We narrowed to 457 results for: Si

1. E1_pENTR_L1-L4_NN_G1397-DddA11'-C

   Plasmid

   #191606

   Purpose

   ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

   Insert

   C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL2080

   Plasmid

   #155160

   Purpose

   Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF_L266A

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2081

   Plasmid

   #155161

   Purpose

   Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF(Y261A_L265A_L266A)

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL1305

   Plasmid

   #155143

   Purpose

   Expresses MlaFB, His tag on MlaF N-term

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   (6xHis-TEV-MlaF)-MlaB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL1306

   Plasmid

   #155144

   Purpose

   Expresses MlaFB, His tag on MlaF C-term

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   (MlaF-6xHis)-MlaB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL1307

   Plasmid

   #155145

   Purpose

   Expresses MlaFB, His tag on MlaB N-term

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL1308

   Plasmid

   #155146

   Purpose

   Expresses MlaFB, His tag on MlaB C-term

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(MlaB-6xHis)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL1833

   Plasmid

   #155149

   Purpose

   Expresses MlaF(1-250)-GCN4

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-250)-GCN4_Dimerization_Domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL1957

   Plasmid

   #155151

   Purpose

   Expresses Strep-MlaF-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   added Streptavidin tag on MlaF N-terminus

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL2074

    Plasmid

    #155155

    Purpose

    Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaB_T52E

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL2076

    Plasmid

    #155156

    Purpose

    Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_K47A

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL2077

    Plasmid

    #155157

    Purpose

    Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_E170Q

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL2078

    Plasmid

    #155158

    Purpose

    Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_Y261A

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL2079

    Plasmid

    #155159

    Purpose

    Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_L265A

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-PEAR-mCherry

    Plasmid

    #228892

    Purpose

    All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Insert

    EGFP-IRES-mScarlet
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYESir2-puro

    Plasmid

    #1769

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-GINKO1

    Plasmid

    #113112

    Purpose

    Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-TA-ERN

    Plasmid

    #80474

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TAC-ERN

    Plasmid

    #80475

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TAG-ERN

    Plasmid

    #80476

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-TAC-ERP2

    Plasmid

    #80478

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-NES-KIRIN1

    Plasmid

    #107416

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Rc/CMV cyclin E

    Plasmid

    #8963

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin E (CCNE1 Human)
    

    Use

    Tags

    GPI linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PB-TAG-ERP2

    Plasmid

    #80479

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-KIRIN1-GR

    Plasmid

    #107418

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1-GR

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BPTI-[5,55]Gly14Ile38

    Plasmid

    #195059

    Purpose

    Thermal stability of BPTI

    Depositor

    Yutaka Kuroda

    Article

    Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

    Insert

    bovine pancreatic trypsin inhibitor
    

    Use

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    Changed Alanine residues at position of 14 and 38…

    Promoter

    T7 promotor

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. aCKAR

    Plasmid

    #124427

    Purpose

    FRET-based reporter for monitoring aPKC activity in cells

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

    Insert

    aCKAR
    

    Use

    Tags

    mCFP and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Rc/CMV cyclin C

    Plasmid

    #8961

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin C (CCNC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pRK-flag-USP19 C506S

    Plasmid

    #78584

    Purpose

    Expression flag-USP19 C506S in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 C506S (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 506 cysteine residue to serine

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

31. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pBS-KSTbx2.8.2

    Plasmid

    #42626

    Depositor

    Virginia Papaioannou

    Article

    Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

    Insert

    Tbx2 (Tbx2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pAAV-ND4 Right-GSVG-UGI

    Plasmid

    #187414

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-ND4 Right-E1347A-UGI

    Plasmid

    #187415

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK-flag-GFP 1-10 tetra Cys

    Plasmid

    #78589

    Purpose

    Expression flag-GFP 1-10 tetra Cys in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 1-10 tetra Cys
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pLKO.1-mPRAS40

    Plasmid

    #15480

    Depositor

    Do-Hyung Kim

    Article

    Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

    Insert

    PRAS40 shRNA (Akt1s1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCitrine-USP19 1-1290

    Plasmid

    #78595

    Purpose

    Express mCitrine-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

40. PX543 SpCas9(del175-307)

    Plasmid

    #58888

    Purpose

    Truncated form of SpCas9 without REC2 domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 175-307

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PB-TAC-Cas9

    Plasmid

    #120352

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PB-TAC-KRAB-dCas9

    Plasmid

    #120355

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBEL2082

    Plasmid

    #155162

    Purpose

    Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    (MlaFΔ[248-269])-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 248-269 in MlaF

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBEL1514

    Plasmid

    #155147

    Purpose

    Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBEL1965

    Plasmid

    #155152

    Purpose

    Expresses MlaF(1-246)-(6 aa linker)-GCN4

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBEL1966

    Plasmid

    #155153

    Purpose

    Expresses MlaF(1-246)-(10 aa linker)-GCN4

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBEL1967

    Plasmid

    #155154

    Purpose

    Expresses MlaF(1-246)-(14 aa linker)-GCN4

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. CAG-Avr-HPRT-RIGHT

    Plasmid

    #107272

    Purpose

    Expresses TALEN (right) targeting HPRT exon3

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    HPRT1_B Avr-TALEN (right)
    

    Use

    TALEN

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. CAG-Avr-HPRT-LEFT

    Plasmid

    #107271

    Purpose

    Expresses TALEN (left) targeting HPRT exon3

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    HPRT1_B Avr-TALEN (left)
    

    Use

    TALEN

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge