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Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-S653Stop
Plasmid#31976DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFPExpressionMammalianMutationchanged Serine 653 to Stop codonPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-Q735Stop
Plasmid#31977DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFPExpressionMammalianMutationchanged Glutamine 735 to Stop codonPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-D299A
Plasmid#31975DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationchanged Aspartic acid 299 to AlaninePromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-pDisplay-SNAP[C145A]
Plasmid#223496PurposeExpression of dead-mutant SNAP-tags to outer surface in mammalian cellsDepositorInsertdead-mutant SNAP-tag
UseTagsExpressionMammalianMutationSNAP-tag C145APromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh-EGFR
Plasmid#208280PurposeExpresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-EGFR
UseLentiviralTagsExpressionMutationH225N in mCherry- please see depositor commentPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPL1B-Cl
Plasmid#160430PurposeExpresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoterDepositorInsertRPL1B
UseTagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to vary nucleoti…PromoterS. cerevisiae GAL1Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
UseTagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL800
Plasmid#119306Purposeprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290SDepositorInsertprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
UseTagsnoneExpressionBacterialMutationamino acids 1 through 171 deleted, and mutatioms …PromoterlacAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
UseTagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-pur-CAG-mCherry
Plasmid#159458PurposeAAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection geneDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1-IRES2-EGFP
Plasmid#170820Purposehuman GABAA receptors alpha1 subunit and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMCP-Luc (MCP-1 promoter in pGL3-basic)
Plasmid#40324DepositorInsertMCP-1 promoter (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-mOXT-hM3Dq-mCherry-WPRE
Plasmid#70717PurposeExpresses DREADDs Gq receptor under oxytocin promoter controlDepositorInserthM3Dq-mCherry
UseAAVTagsmCherryExpressionMutationPromotermouse oxytocinAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LAMP1
Plasmid#110948PurposeExpresses LAMP1 fused to mTurquoise2DepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_mKate2
Plasmid#96942PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertfar-red fluorescent protein
UseLentiviralTagsExpressionMutationE87D (please see depositor comments below)PromoterCaMKIIaAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pSLIK TT FLAG-HA RBFOX2
Plasmid#59769Purposedoxycycline-inducible expression of FLAG-HA-tagged human RBFOX2 in mammalian cellsDepositorInsertRBFOX2 (RBFOX2 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterTRE tightAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Th-tdTomato
Plasmid#159460PurposeTargeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassetteDepositorInsertP2A-tdTomato
UseCre/LoxTagsP2AExpressionMammalianMutationPromoterEndogenous THAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-NG1Tt
Plasmid#44523DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPGal1 (wt)Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-K225insNM
Plasmid#31974DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationinserted amino acids Asn-Met before Lys225PromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Cyp26a1
Plasmid#185563PurposeAAV construct to ubiquitously express mouse Cyp26a1DepositorInsertCyp26a1 (Cyp26a1 Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-F112S
Plasmid#48727PurposeBacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationPhenylalanine 12 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-I98S
Plasmid#48723PurposeBacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 98 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-I73S
Plasmid#48722PurposeBacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 73 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-V69S
Plasmid#48721PurposeBacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationValine 69 to SerinePromoterT7Available sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL
Plasmid#34593DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pTH-iCre:EGFP-WPREpA
Plasmid#213142PurposeTruncated rat TH promoter expressing iCre fused to EGFPDepositorInsertiCre
UseAAVTagsEGFPExpressionMammalianMutationPromoterTruncated TH promoter from rat (Rattus norvegicus…Available sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-HA
Plasmid#49851PurposeEncodes human connexin 43 with a C-terminal HA epitope fusion tagDepositorInsertConnexin 43 (GJA1 Human)
UseTagsHAExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOS003-lenti-CMV-GCaMPer
Plasmid#65227PurposeER calcium sensor for lentivirus productionDepositorInsertGCaMPer
UseLentiviralTagscircularly permuted GFPExpressionMammalianMutationGCaMP3 (D324G, D360G, D397G, D435G)PromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pTH-iCre-WPREpA
Plasmid#213130PurposeTruncated rat TH promoter expressing iCreDepositorInsertiCre
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationPromoterTruncated TH promoter from rat (Rattus norvegicus…Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX-SP-YFP-STIM1(23-685)
Plasmid#18857DepositorInsertStromal interaction molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationYFP is inserted between residues 1-23 (signal pep…PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
UseTagsExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T373
Plasmid#58912Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with T373 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S612
Plasmid#58914Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S612 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S608
Plasmid#58913Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S608 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PQBP1
Plasmid#25804DepositorInsertPQBP1 (PQBP1 Human)
UseTags6xHAExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEX-SP-CFP-STIM1(23-685)
Plasmid#18858DepositorInsertStromal interaction molecule 1 (STIM1 Human)
UseTagsCFPExpressionMammalianMutationCFP inserted between residues 1-23 (signal peptid…PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag-Msi1
Plasmid#184029PurposeMsi1 clone with N-terminal flag tagDepositorInsertMsi1 (Msi1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16–RAR-alpha
Plasmid#80434PurposeExpression of constitutively active human RAR-alpha in chick embryosDepositorInsertRetinoic acid receptor alpha (RARA Human)
UseTagsEGFP and VP16ExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAd5-Blue
Plasmid#174431PurposepAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.DepositorInsertβ-galactosidase α gene fragment
UseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD21-hgAPOAI
Plasmid#124962PurposeExpress ApoAI in the liverDepositorInserthuman APOAI gene (LPA Human)
UseAdenoviralTagsExpressionMutationPromoterhuman APOAIAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-siResist
Plasmid#49852PurposeEncodes human connexin 43 with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Neurod1-Atoh7HA3
Plasmid#44438DepositorInsertAtoh7-3xHA knock-in (Atoh7 Mouse)
UseCre/Lox and Mouse TargetingTagsNeurod1 left arm, Neurod1 right arm, and loxC2-Re…ExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S780
Plasmid#58915Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S780 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Gh
Plasmid#44507DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
Plasmid#44597DepositorInsertEF1a-GFP:2A:CHER(HA)
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2528-3971)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only