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Plasmid#184932PurposeCodon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmidDepositorInsertElectra1
UseTagsExpressionWormMutationPromotertag-168Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP713-T2A-HO1
Plasmid#197246PurposeExpresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegansDepositorInsertwmiRFP713-T2A-HO1
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM3 = ncs-1p::nCre
Plasmid#24034DepositorInsertncs-1 (ncs-1 Nematode)
UseCre/LoxTagsnCreExpressionWormMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCFJ210 - pDESTR4-R3(ttTi4348, I)
Plasmid#34863DepositorInsertttTi4348 targeting
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceJune 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBN154
Plasmid#86718PurposeFLP/Frt dual color reporter for C. elegansDepositorInsertPhsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
UseTagsExpressionWormMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-GFP-RGG
Plasmid#124948PurposeExpression of RGG-GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsEGFP and HisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFF-1::V5
Plasmid#132961PurposeExpresses EFF-1 A full length protein in mammalian cellsDepositorInsertEFF-1::V5
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
BSP610
Plasmid#122255Purposeexpression vector for mtagBFP2 under EFT3 promoterDepositorInsertmtagBFP2
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3011
Plasmid#122539PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::cup-5 (cup-5 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOD2048-csnDEG
Plasmid#89368PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPosm-6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TC374
Plasmid#122614PurposePrpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
UseTagsExpressionWormMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1586
Plasmid#121057PurposemKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmKate2-T^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized mKate2, and au…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3003
Plasmid#122537PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::pisy-1 (pisy-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-set2
Plasmid#82546PurposePmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::set-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT3014
Plasmid#122540PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::rab-7 (rab-7 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
Plasmid#182357PurposePositive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.DepositorInsertGFP
UseTagsExpressionWormMutationPromoterPeft-3Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS002
Plasmid#188325PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::GFP::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-B
Plasmid#177785PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM3814
Plasmid#154122Purposemec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vectorDepositorInsertmec-4p::rtetR-L-QFAD::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM246 - MCS mosTI unc-119 target
Plasmid#159821PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertMCS mosTI unc-119 target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_aman-2::CemOrange2 (aman-2 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Plasmid#159849PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJA58
Plasmid#59933PurposegRNA for cleavage at dpy-10(cn64) locus in C elegansDepositorInsertdpy-10(cn64) gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR49
Plasmid#69259Purposephlh-8::tagBFP2DepositorInsertTagBFP2
UseCre/LoxTagsExpressionWormMutationcodon-optimzed index 1.0, TagBFP2 contains 2 sing…Promoterhlh-8Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT3024
Plasmid#122542PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_mt::CemOrange2
UseTagsMLSLRQSIRFFKPATRTLCSSRTLLExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3007
Plasmid#122538PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::rab-5 (rab-5 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJS008
Plasmid#188331PurposePCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::wrmScarlet::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM3724
Plasmid#154112PurposetetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vectorDepositorInserttetO 7X::∆pes-10::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT3037
Plasmid#122544PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_sqst-1::CemOrange2 (T12G3.1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159857PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCG142
Plasmid#17246DepositorInsertpie-1 intron:pie-1 promoter in PDONRP4P1R
UseGatewayTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
ChrII_ttTi5605_loxP_myo2_neoR
Plasmid#194056PurposeDual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genomeDepositorInsertttTi5605
UseTagsExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJJS006
Plasmid#188329PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mNeonGreen::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWP018
Plasmid#198766PurposeDrives specific expression in the ADE, CEP, and PDE neuronsDepositorInsertPdat-1-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS235
Plasmid#191855PurposeNegative selection marker for MosSCIDepositorInsertpeel-1 toxic protein for negative selection against extrachromosomal array
UseTagsExpressionWormMutationPromoterhsp-16.41Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
ChrV_oxTi365_loxP_myo2_neoR
Plasmid#194057PurposeDual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genomeDepositorInsertoxTi365
UseTagsExpressionWormMutationPromoterNoneAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJS005
Plasmid#188328PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mNeonGreen::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
Plasmid#159814PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCFJ108 - pENTR221 - [cbr-unc-119(+)]
Plasmid#200367Purposecbr-unc-119 rescue gene for use as co-injection marker.DepositorInsert[cbr-unc-119(+)]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pflp-18::NGL-1::spGFP11
Plasmid#65828PurposeFor trans-synaptic labelingDepositorInsertNLG-1 (nlg-1 Nematode)
UseTagsspGFP11ExpressionWormMutationN142SPromoterPflp-18Available sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1839
Plasmid#154325PurposesgRNA Destination Vector for SapTrapDepositorInsertSapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSiM1
Plasmid#199380PurposeEncodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD288
Plasmid#137017PurposemNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized mNGExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorInsertgfp11::rps-18 (rps-18 Nematode, Synthetic)
UseGatewayTagsGFP11ExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BSP188
Plasmid#110917PurposeFor cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator clonedDepositorInsertunc54 Terminator
UseSynthetic Biology; Yeast homologous recombination…TagsExpressionWormMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only