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Plasmid#191108PurposeMammalian cell line expression plasmids for production of EGFP and FusionRed proteinsDepositorInsertpAAV-CAG-EGFP-P2A-FusionRed
UseTagsFusionRedExpressionMammalianMutationSee depositor comments belowPromoterchicken β-actin promoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-CBh-aSyn-WPRE3-enSV40pA
Plasmid#194244PurposeExpresses human alpha-Synuclein WTDepositorInsertaSynuclein(WT) (SNCA Human)
UseAAVTagsExpressionMutationPromoterCBhAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd
Plasmid#118278PurposeExpresses bPAC(WT)-mCherry under a minimal CamKII PromoterDepositorInsertbPAC(WT)
UseAAVTagsmCherry and mycExpressionMutationPromoterCamKIIAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-dsRed-shvgat
Plasmid#67845PurposeExpresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switchDepositorInsertAAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiTagsExpressionMammalianMutationPromoterh-synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoExpressionMutationPromoterCAGAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Chronos-tdTomato]
Plasmid#84481PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDi-P2A-mCherry
Plasmid#204358PurposeAAV vector for miniDi expression under the control of human synapsin promoterDepositorInsertminiDi-P2A-mCherry
UseAAVTagsmCherryExpressionMutationThe third intracellular loop (ICL3) of hM4Di was …PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
161_pAAV-ProD5-CatCh-GFP-WPRE
Plasmid#125981PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProD5Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN-miRFP670nano3-WPRE-hGHpA
Plasmid#184688PurposeMammalian expression, miRFP670nano3DepositorInsertmiRFP670nano3
UseAAVTagsExpressionMutationPromoterhSYNAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-RiboL1-jGCaMP8m
Plasmid#192617PurposeAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.DepositorInsertFLEX-RiboL1-jGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
113_pAAV-ProC21-CatCh-GFP-WPRE
Plasmid#125956PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProC21Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
Plasmid#65418PurposeAAV packaging vector for CamKIIa driven KOR DREADD expressionDepositorInsertCaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
UseAAVTagsHA and mCitrineExpressionMutationPromoterCaMKIIaAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon EYFP
Plasmid#55650PurposeCre-on/Flp-on EYFP under the Synapsin promoterDepositorHas ServiceAAV Retrograde and AAV8InserteYFP with introns
UseAAV; Cre on/flp on eyfpTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eGFP-2A-TetanusToxin
Plasmid#166603PurposeEncodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TREDepositorInsertEGFP-2A-Tetanus Toxin
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-biPAC
Plasmid#169127PurposeAAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPACDepositorInsertmKate2-biPAC
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationcodon optimized to mousePromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-stGtACR2-FusionRed
Plasmid#217975PurposeAAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent mannerDepositorInsertstGtACR2-FusionRed
UseAAVTagsFusionRedExpressionMutationPromoterEF1aAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry
Plasmid#166599PurposeEncodes Cre-dependent hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hMECP2-cycle3GFP
Plasmid#163706PurposepAAV plasmid for Cre-dependent expression of human MECP2 fused with cycle3 GFP under Syn promoterDepositorInserthMECP2-cycle3GFP (MECP2 Human, Aequorea victoria)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniGFP1-P2A-FusionRed
Plasmid#188732PurposeExpress miniGFP1 and FusionRed in mammalian cellsDepositorInsertminiGFP1
UseAAVTagsFusionRedExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE
Plasmid#183522PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet
UseAAVTagsExpressionMutationI146M/G174SPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-Ef1a-TVA-2A-N2cG
Plasmid#172360PurposeTargeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-TdTomato
Plasmid#191203PurposeFlpO dependent TdTomato expressionDepositorInsertTdTomato
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hChR2(H134R)-EYFP
Plasmid#27054PurposeAAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitationDepositorInserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationH134RPromoterGFAPAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ConVERGD-eGFP-WPRE
Plasmid#218749PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA
Plasmid#216319PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Cerulean + splice acceptor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA
Plasmid#216318PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertsplit cerulean fluorescent protein + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eNpHR 3.0-EYFP
Plasmid#26972PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibitionDepositorHas ServiceAAV5InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iRFP682-P2A-GFP
Plasmid#197153PurposeExpresses the protein of iRFP682-P2A-GFP in mammalian cellsDepositorInsertiRFP682-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)
Plasmid#209198PurposeMutagenesis of ThDepositorInsertTh (Th Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterCMVAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CjCas9-eGFP-HIF1a
Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAV and CRISPRTagsSV40NLS-HA-T2A-EGFPExpressionMutationPromoterEF-1alphaAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-eGFP-WPRE
Plasmid#218743PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-oG-WPRE-SV40pA
Plasmid#74291PurposepAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVTagsExpressionMammalianMutationchimeric glycoproteinPromoterCAGAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGluSnFR4f-PDGFR-WPRE
Plasmid#234450PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-PDGFR
UseAAVTagsIgK chain and Myc epi tag-PDGFR TM DomainExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOTTC385 - pAAV CMV-IE IRES EGFP
Plasmid#102936PurposeAn AAV vector that expresses IRES EGFP under the CMV-IE promoterDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniGFP2-P2A-FusionRed
Plasmid#188734PurposeExpress miniGFP2 and FusionRed in mammalian cellsDepositorInsertminiGFP2
UseAAVTagsFusionRedExpressionMammalianMutationPromoterCAGAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-Cas9C-P2A-turboGFP
Plasmid#80942PurposeExpresses Cas9C in mammalian cells; co-expresses turboGFP.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-ChR2-mCherry-Fishell-3
Plasmid#83898PurposeChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertChR2
UseAAVTagsExpressionMammalianMutationPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Flag-PGC1a-6His
Plasmid#67637PurposeAAV vector expressing PGC1a geneDepositorInsertPGC1a (Ppargc1a Mouse)
UseAAVTags6XHis and Flag tagExpressionMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-DIO-PdCO-mScarlet-WPRE
Plasmid#198511PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterpCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGW03 AAV-TBG-Cre-sgRNA
Plasmid#192163PurposeAAV-TBG-Cre-sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#125692PurposeGlobal labeling of inhibitory synapses (green fluorescence)DepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-intron2-dLUC
Plasmid#110059PurposePer2 transcription luciferase reporterDepositorInsertPer2 promoter and luciferase (Per2 Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterPer2Available sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA
Plasmid#185715PurposeAAV expression of GFP and human α-Synuclein from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry
Plasmid#50458PurposeDouble floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas ServiceAAV8InsertrM3D-mCherry
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-mScarlet
Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-moxGFP
Plasmid#227273PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only