We narrowed to 29,387 results for: REP

1. pCD5-PR8-TEV-sfGFP-twinStrep

   Plasmid

   #157933

   Purpose

   Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

   Insert

   A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
   

   Use

   Tags

   sfGFP & TwinStrep

   Expression

   Mammalian

   Mutation

   E190D

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. T7 promoter + filler DNA reporter plasmid

   Plasmid

   #156454

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-28-Strep-OPA1(MGD) (SB264)

   Plasmid

   #227613

   Purpose

   Inducible expression of Strep-tagged Opa1(MGD) in E.coli

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Tags

   Twin-StrepTag

   Expression

   Bacterial

   Mutation

   contains aa 253-580 (MGD) and aa 938-960

   Promoter

   T7

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-AU1

   Plasmid

   #162113

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-HA-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-HA-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-FLAG

   Plasmid

   #162092

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-HA-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-HA-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-TWIN-STREP-FLAG-NPRL2

   Plasmid

   #164985

   Purpose

   NPRL2 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   NPRL2 (NPRL2 Human)
   

   Use

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xFlag-tdMCP-Erk2 (pErk reporter)

   Plasmid

   #226167

   Purpose

   Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-Erk2 (MAPK1 Human)
   

   Use

   Tags

   3xFlag-tdMCP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-6xGLY-Strep-tagII-HIS3MX6

   Plasmid

   #20756

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid

    #197872

    Purpose

    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Inserts

    part kilA/repL
    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains only the 24 bp of 3'-end of kilA ge…

    Promoter

    Bba_J23115 and PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFl Strep-sumo-TEV-hAgo2

    Plasmid

    #136235

    Purpose

    Expresses human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Use

    Tags

    Strep Sumo

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA

    Plasmid

    #162109

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-HA
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-HA

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1

    Plasmid

    #162093

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-HA-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-HA-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc

    Plasmid

    #161585

    Purpose

    expression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutation

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-TWIN-STREP-FLAG-NPRL3

    Plasmid

    #164984

    Purpose

    NPRL3 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    NPRL3 (NPRL3 Human)
    

    Use

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52379

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    OCT4

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-mTagBFP2-TEV-Strep-His

    Plasmid

    #210824

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mTagBFP2
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-K27 histone methylation reporter

    Plasmid

    #22865

    Depositor

    Alice Ting

    Article

    Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

    Insert

    K27 HMR
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis(TEV)-Drp1(K38A)-StrepII

    Plasmid

    #174423

    Purpose

    Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K38A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge