We narrowed to 22,868 results for: REP

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pMB1610_pRR-Puro

Plasmid

#65853

Purpose

Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split puromycin N-acetyltransferase coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRN110

Plasmid

#84462

Purpose

pJB38-NWMN29-30 + SarA_P1-mKate2-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mKate2 from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-1

Plasmid

#61736

Purpose

Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

Depositor

Huimin Zhao

Article

Cobb et al ACS Synth Biol. 2014 Dec 8.

Inserts

sSpCas9
tracrRNA
crRNA cassette

Use

CRISPR

Expression

Bacterial

Mutation

BbsI-flanked lacZ cassette inserted in place of s…

Promoter

gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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hiActTS-GR

Plasmid

#79775

Purpose

high FRET control for ActTS-GR

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

hiActTS-GR

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP6

Plasmid

#18065

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

Plasmid

#225438

Purpose

Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-FRT-OCS-BeYDVRepRepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCRZero

Plasmid

#120275

Purpose

Blunt-end cloning vector of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Article

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Synthetic, Human)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

Plasmid

#64221

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ223 (dAsCpf1-SRDX)

Plasmid

#86209

Purpose

Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

dAsCpf1

Use

Gateway compatible cpf1 vectors

Tags

NLS and SRDX repressor and NLS

Expression

Plant

Mutation

D908A

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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hNET pcDNA3

Plasmid

#15475

Depositor

Randy Blakely

Article

Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

Insert

hNET (SLC6A2 Human)

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowG

Plasmid

#104620

Purpose

Expresses Shadow G in mammalian cell

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

Insert

ShadowG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pTagRFP675-N1

Plasmid

#44275

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

Plasmid

#138418

Purpose

CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Promoter

EF1a

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-mPERK-FLAG-IP

Plasmid

#101793

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PERK

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Perk (Eif2ak3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-wtSrc

Plasmid

#140295

Purpose

Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Tags

2X Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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