20,081 results

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBB528

Plasmid

#27390

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

lacIq

Expression

Bacterial

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-BICDN-CIBN

Plasmid

#102254

Purpose

Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

GFP-BICDN-CIB1 (CIB1 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

F9 promoter

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-CaURA3

Plasmid

#44872

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-IRES-HO-1

Plasmid

#80345

Purpose

Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Active Wnt10B-V5

Plasmid

#43823

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT10B (WNT10B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pOpen-P45

Plasmid

#165570

Purpose

Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

P45

Use

Synthetic Biology

Available Since

Aug. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pmCherry_a_tubulin_IRES_puro2

Plasmid

#21043

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pHSN6I01

Plasmid

#50587

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

dCas9-KRAB
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG, HA, and NLS

Mutation

dCas9 that is defective in DNA cleavage; the maiz…

Promoter

2×35Sp and AtU6-26p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTpPuc3

Plasmid

#62864

Purpose

Episome vector for Thalassiosira pseudonana (pUC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for thalassios…

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

Plasmid

#55201

Purpose

Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dsRed2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-FOXO3

Plasmid

#153142

Purpose

Expression of Flag tagged human FOXO3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO3 (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-CRKL

Plasmid

#29560

Depositor

Nora Heisterkamp

Article

Fioretos et al Oncogene. 1993 Oct . 8(10):2853-5.

Insert

CRKL (CRKL Human)

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
S-HBsAg

Plasmid

#103013

Purpose

Expresses HBV Small envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Small envelope protein

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAS1NB c Rosella I

Plasmid

#71245

Purpose

a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

Depositor

Mark Prescott

Article

Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

Insert

Rosella

Expression

Yeast

Promoter

PGK1

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen

Plasmid

#99131

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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SNAP-Drp1

Plasmid

#141159

Purpose

Mammalian expression and labeling of Drp1

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

DRP1 (DNM1L Human)

Tags

SNAP-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MBX

Plasmid

#64122

Purpose

a modified episomal (EBNA1/OriP) vector expressing human BCL2L1 (BCL-xL) gene

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

BCL2L1 (BCL2L1 Human)

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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