We narrowed to 17,543 results for: Emb
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EGFP-Rab4aM-7
Plasmid#56441PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab4a (RAB4A Human)
TagsEGFPExpressionMammalianMutationMutation removes NheI sitePromoterCMVAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTNos:BASTA:PNos (GB0023)
Plasmid#68211PurposeProvides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.DepositorInsertbar gene
UseUnspecifiedMutationBsaI and BsmBI sites removedPromoterPNosAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-deltaY20
Plasmid#58459PurposeExpresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 20 deletedPromoterCMV IEAvailable SinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
B0032m_BC
Plasmid#66020PurposeMoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]DepositorInsertRBS
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
WPXL LEFTY2 gRNA
Plasmid#101924PurposeLEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.DepositorInsertLEFTY2 enhancer targeting gRNA
UseLentiviralAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S-HLx8
Plasmid#108138PurposeRecruitable inactive SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-Y20A
Plasmid#58460PurposeExpresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB1
Plasmid#131158PurposeRetroviral expression of flag-tagged mouse TRIB1DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66064PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterU6-26Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTV-mHdac1-tdiRFP670-PEST2
Plasmid#122351PurposeTargeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.DepositorInsert2A-tdiRFP-PEST with homology arms for Hdac1
UseMouse TargetingAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sema4a-AP-His
Plasmid#72028PurposeExpresses the extracellular region of the Sema4A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-NHR2 pcDNA3.1
Plasmid#17318DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
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mCit-Snap25b D179K A199R
Plasmid#53259PurposeSNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
Tags4aa-linker and mCitrineExpressionMammalianMutationD179K and A199RPromoterCMVAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT
Plasmid#61792PurposeLuciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutatedDepositorInsertmouse Ywhag 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only
