165,688 results

1. pCR4-24XPP7SL

   Plasmid

   #31864

   Depositor

   Robert Singer

   Article

   Larson et al Science. 2011 Apr 22;332(6028):475-8.

   Insert

   24xPP7 Stem Loop Cassette
   

   Available Since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTFP1-pBAD

   Plasmid

   #54553

   Purpose

   Localization: Protein Expression Vector , Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

   Type

   Empty backbone

   Tags

   6xHis and mTFP1

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

   Plasmid

   #196415

   Purpose

   Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smMyc
   

   Use

   AAV; Astrocyte-selective

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNL-CEF-BAFFR-CFP

   Plasmid

   #187002

   Purpose

   Expression of human BAFFR-CFP fusion protein

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   BAFFR (TNFRSF13C Human)
   

   Use

   Lentiviral

   Tags

   CFP

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET.HaloTag.v7

   Plasmid

   #226197

   Purpose

   bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti‐Cas9‐2A‐Blast

   Plasmid

   #73310

   Purpose

   Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIND-HA-ADH

   Plasmid

   #11991

   Depositor

   Steven Johnson

   Insert

   aldehyde dehydrogenase (ALDH1A1 Human)
   

   Use

   Ecdysone inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSynapsin1.OxLight1

    Plasmid

    #169792

    Purpose

    Fluorescent reporter for orexin neuropeptides

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

    Insert

    OxLight1
    

    Use

    AAV

    Promoter

    human Synapsin-1

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-SYN-sDarken

    Plasmid

    #184796

    Purpose

    Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-TetON-Puro-HIF1a 0819

    Plasmid

    #118704

    Purpose

    Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    shRNA against human HIF1a
    

    Use

    Lentiviral

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-KASH2ext

    Plasmid

    #187018

    Purpose

    Control for inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2ext
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-V5-SBP-C1-HshnRNPK

    Plasmid

    #64923

    Purpose

    Expresses V5- and SBP-tagged hnRNPK in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPK (HNRNPK Human)
    

    Tags

    V5 and SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-N-Zeo-mStayGold

    Plasmid

    #227252

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Mfn1

    Plasmid

    #141154

    Purpose

    Mammalian expression and labeling mitofusin 1

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 1 (MFN1 Human)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHJL3

    Plasmid

    #199378

    Purpose

    Encodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    jGCaMP7s::SL2::mScarlet-I
    

    Expression

    Bacterial

    Mutation

    no mutations

    Available Since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. CL20_mEGFP_KMT2A_MLLT1

    Plasmid

    #205842

    Purpose

    Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KMT2A_MLLT1 (KMT2A, MLLT1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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