162,999 results

1. pCW-HA-hRb-WT-puro

   Plasmid

   #212672

   Purpose

   Express tagged hRb

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 (+) -myc-OGA(GS-544)C181

   Plasmid

   #193996

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBad-CA

   Plasmid

   #105665

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Man2a1-GFP

   Plasmid

   #163649

   Purpose

   Expresses GFP tagged Man2a1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mannosidase alpha class 2A member 1 (MAN2A1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutation was made to introduce a XbaI rest…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194284

   Purpose

   EF1a-dCas9-KRAB-GFP with nontarget sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-FLAG-BMAL1

   Plasmid

   #186829

   Purpose

   Expresses FLAG-BMAL1 in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV_EBOV_GP

   Plasmid

   #207271

   Purpose

   Mammalian expression of Ebola virus GP protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   EBOV_GP (GP Ebola virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-Flag-BRD4-7D

   Plasmid

   #90007

   Purpose

   expresses 7D mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 484S to 484D, 488S to 488D, 492S to 492D,…

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRX-IB-HaloTag7-mGFP

   Plasmid

   #184903

   Purpose

   Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   monomeric EGFP
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMU-PERr

    Plasmid

    #61202

    Purpose

    Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    mCherry-SKL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdTomato-H2B-6

    Plasmid

    #58101

    Purpose

    Localization: Nucleus/Histones, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28C-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204402

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAEMXT-eLACCO1.1

    Plasmid

    #167946

    Purpose

    Fluorescent biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    CD59-eLACCO1.1-GPI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. HRE-oUnaG

    Plasmid

    #201712

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-NEDD8

    Plasmid

    #18711

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    NEDD8 (NEDD8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191182

    Purpose

    Expression of EACTB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:ACTB.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Noggin

    Plasmid

    #163586

    Purpose

    Expresses Noggin in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Nog (Nog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ubiquitin (1-76; T66V, L67N)

    Plasmid

    #110755

    Purpose

    Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T66V, L67N

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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