161,255 results

1. pIG-704_CD19-28z_CAR_tNGFR

   Plasmid

   #207486

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, CD19-28z_CAR (NGFR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pReporter_8

   Plasmid

   #60568

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

   Plasmid

   #137993

   Purpose

   Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDual_dsCas9_BFP

   Plasmid

   #214679

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-Precission-NEDD4L_FL_pCDNA3.1(+)

   Plasmid

   #187730

   Purpose

   Express NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TNFR1-YFP

   Plasmid

   #111209

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Insert

   TNFRSF1A (TNFRSF1A Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIntegrase_4

    Plasmid

    #60576

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRN3P-HA-Suv4-20h1

    Plasmid

    #86689

    Purpose

    For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mutation

    Promoter

    T3

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM5087

    Plasmid

    #85123

    Purpose

    Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GPR161-Tango

    Plasmid

    #66334

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR161 (GPR161 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pQCXIZ 3xFLAG-TREX1

    Plasmid

    #164243

    Purpose

    retroviral plasmid for 3xFLAG-TREX1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIGR1-HA-mTak1

    Plasmid

    #174740

    Purpose

    Gene overexpression

    Depositor

    Shao-Cong Sun

    Insert

    Tak1 (Nr2c2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-8xHis-Ub

    Plasmid

    #107392

    Purpose

    Mammalian expression 8xHis-Ub

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    8 tandem repeats of 6xHis-Ub (See comments below)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRN3P-HA-Suv4-20h2

    Plasmid

    #86691

    Purpose

    For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTAL-MajSat-FLAG-NLS-VP64

    Plasmid

    #69074

    Purpose

    Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    TALE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

    Plasmid

    #114322

    Purpose

    Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge