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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHM6-alphasynuclein-A53T

Plasmid

#40825

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNCA (SNCA Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

A53T

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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RCIscript-GoldyTALEN

Plasmid

#38142

Purpose

destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

Depositor

Daniel Carlson, Stephen Ekker

Publication

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Mrna transcription vector

Tags

AcV5 and FokI homodimer

Mutation

Truncate at N and C terminous

Promoter

T3

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pGL2-p21 promoter-Luc

Plasmid

#33021

Depositor

Martin Walsh

Publication

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p21 promoter (CDKN1A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

p21

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Publication

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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FUW-tetO-hKLF4

Plasmid

#20725

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Publication

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Available since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Publication

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pPGKpuro

Plasmid

#11349

Depositor

Rudolf Jaenisch

Publication

Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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rAAV2/MG1.2

Plasmid

#184541

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.2 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, Q588T; insertion o…

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHR-FUSN-mCh-Cry2WT

Plasmid

#101223

Purpose

FUS(1-214) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLenti-Cas9-GFP

Plasmid

#86145

Purpose

Lentiviral vector expressing Cas9-GFP

Depositor

David Sabatini

Publication

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

Cas9-GFP

Use

CRISPR and Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Promoter

EFS

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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mEos3.2-C1

Plasmid

#54550

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson, Tao Xu

Publication

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Tags

mEos3.2

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

20 Articles

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Publication

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pHM6-alphasynuclein-WT

Plasmid

#40824

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNCA (SNCA Human)

Tags

6x His and HA

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA-4mtD3cpv

Plasmid

#36324

Depositor

Amy Palmer, Roger Tsien

Publication

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria

Tags

First 29 aa of human COX subunit 8a

Promoter

CMV

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Publication

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCMV-GAL4

Plasmid

#24345

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAL4

Expression

Mammalian

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-hAgo2

Plasmid

#21981

Depositor

Phil Sharp

Publication

Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Argonaute 2 (AGO2 Human)

Expression

Mammalian

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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HA-HIF2alpha-P405A/P531A-pcDNA3

Plasmid

#18956

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pcDNA3-TLR2-YFP

Plasmid

#13016

Depositor

Doug Golenbock

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Toll-Like Receptor 2 (TLR2 Human)

Tags

YFP

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Publication

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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sfGFP-pBAD

Plasmid

#54519

Purpose

Localization: Protein Expression Vector , Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Publication

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

6xHis and SfGFP

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

14 Articles

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pLXSN16E6E7

Plasmid

#52394

Purpose

The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

Depositor

Denise Galloway

Publication

Halbert et al J Virol. 1991 Jan;65(1):473-8. doi: 10.1

Insert

HPV16 E6E7

Use

Retroviral

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pPAmCherry1-N1

Plasmid

#31928

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PAmCherry1

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

cmv

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Publication

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Publication

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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SRa-HA-SUMO2 (Sentrin 3)

Plasmid

#17360

Purpose

Mammalian expression of human SUMO2 with HA tag

Depositor

Edward Yeh

Publication

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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