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Addgene

We narrowed to 22,078 results for: his

Showing: 2501 - 2550 of 22078 results
  1. pTT3-ecdCD80-ST3-His

    Plasmid
    #210665
    Purpose
    Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Insert
    Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. CCM2(413-438)_pHisSUMO(K0)

    Plasmid
    #224065
    Purpose
    Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site
    Depositor
    Insert
    ccm2 (CCM2 Human)
    Use
    Tags
    6xHIS; SUMO(K0)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

    Plasmid
    #205052
    Purpose
    Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli
    Depositor
    Insert
    GFP(-6)-H2_mCherry(-6)-L3
    Use
    Tags
    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pRSF-Duet-C17orf53-6His

    Plasmid
    #211417
    Purpose
    bacterial expression of human C17orf53 C-terminally fused to 6xHis-Tag
    Depositor
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pRSF-Duet-6His-C17orf53

    Plasmid
    #211416
    Purpose
    bacterial expression of human C17orf53 N-terminally fused to a 6xHis-Tag
    Depositor
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pJRM919_WT aa 1-419 His POLI

    Plasmid
    #201445
    Purpose
    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI
    Depositor
    Insert
    DNA polymerase iota (POLI Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Expresses amino acids 1-449 of DNA polymerase iot…
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pCI-Puro_hCOL4A4-Myc-His

    Plasmid
    #198093
    Purpose
    Express hCOL4A4 in mammalian cells
    Depositor
    Insert
    COL4A4 (COL4A4 Human)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 2501 - 2550 of 22078 results