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pT3TS-nCas9n

Plasmid

#46757

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Bacterial

Promoter

T3

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway Cloning

Mutation

M260I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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Cdk2-DN-HA

Plasmid

#1885

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSIN4-CMV-K2M

Plasmid

#21164

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Klf4, cMyc (MYC, KLF4 Human)

Use

Lentiviral; Reprogramming

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TBK1

Plasmid

#20648

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC wt

Plasmid

#19304

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMXs-Oct3/4-IP

Plasmid

#15918

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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Cdk5-DN-HA

Plasmid

#1873

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Tags

HA

Expression

Mammalian

Mutation

D145N, dominant negative

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP670nano3

Plasmid

#184664

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSCR

Plasmid

#17920

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only