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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSIN4-EF2-N2L

Plasmid

#21163

Depositor

James Thomson

Publication

Yu et al Science. 2009 Mar 26.

Insert

Nanog, Lin28 (NANOG, LIN28A Human)

Use

Lentiviral; Reprogramming

Expression

Mammalian

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Publication

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Cdk2-DN-HA

Plasmid

#1885

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway Cloning

Mutation

M260I

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSFFV_sfCherry3C(1-10)

Plasmid

#117482

Purpose

Lentiviral vector for constitutive expression of sfCherry3C(1-10)

Depositor

Bo Huang

Publication

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3C(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

5 substitutions (K45R, G52D, T106A, K182R, N194D)…

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Publication

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Promoter

lacUV5

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pWZL Neo Myr Flag TBK1

Plasmid

#20648

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSIN4-CMV-K2M

Plasmid

#21164

Depositor

James Thomson

Publication

Yu et al Science. 2009 Mar 26.

Insert

Klf4, cMyc (MYC, KLF4 Human)

Use

Lentiviral; Reprogramming

Expression

Mammalian

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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Cdk5-DN-HA

Plasmid

#1873

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Tags

HA

Expression

Mammalian

Mutation

D145N, dominant negative

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Publication

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has service

Cloning Grade DNA

Insert

IRES-EGFP

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Has service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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