We narrowed to 17,086 results for: URE

1. pDEST-FRT-TO-GFP-PALB2 KR

   Plasmid

   #71107

   Purpose

   mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   first 8 Lysine residues mutated to Arginine (K14-…

   Promoter

   CMV/TO

   Available since

   Dec. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

   Plasmid

   #83484

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1048A, H1053A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS416-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158148

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first position of th…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2-GFP-TAG

   Plasmid

   #158131

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Introduced a stop codon in place of the tyrosine …

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Nxph4-HA

   Plasmid

   #128102

   Purpose

   Express HA tagged Nxph4

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nxph4 (Nxph4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

   Plasmid

   #83483

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-1-Skp2 WT

   Plasmid

   #81117

   Purpose

   bacterial expression plasmid of Skp2

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLKO.1P shABCB7_2

   Plasmid

   #102969

   Purpose

   Suppression of ABCB7

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shABCB7_2 (ABCB7 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1P shABCB7_1

   Plasmid

   #102968

   Purpose

   Suppression of ABCB7

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shABCB7_1 (ABCB7 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC031 - RcrCas13a from Rhodobacter capsulatus R121

    Plasmid

    #91921

    Purpose

    Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcrCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

    Plasmid

    #91917

    Purpose

    Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LweCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Nxph4-6A-HA

    Plasmid

    #128103

    Purpose

    Express HA tagged Nxph4-6A

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

    Plasmid

    #25458

    Depositor

    Betty Eipper

    Article

    Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

    Insert

    PAM (Pam Rat)
    

    Use

    Tags

    His6 and PAM signal

    Expression

    Mammalian

    Mutation

    Contains aa 498-820 (PALcc= PAL catalytic core) …

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pET28b – E. coli RecE residues 564-868

    Plasmid

    #104534

    Purpose

    Expresses E. coli RecE residues 564-868

    Depositor

    Charles Bell

    Article

    Zhang et al Structure. 2009 May 13;17(5):690-702. do

    Insert

    RecE residues 564-866 (C-terminal nuclease domain)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC032 - RcdCas13a from Rhodobacter capsulatus DE442

    Plasmid

    #91922

    Purpose

    Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcdCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

    Plasmid

    #91920

    Purpose

    Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcsCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

    Plasmid

    #91913

    Purpose

    Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    CaCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZLblasti-CTR1M154A

    Plasmid

    #53158

    Purpose

    Expresses human CTR1 M154A from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    CTR1 (SLC31A1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    M154A

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

    Plasmid

    #91912

    Purpose

    Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LbnCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSKB3-SaGGR-I206F-L377H (JBEI-7202)

    Plasmid

    #110152

    Purpose

    pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

    Depositor

    Jay Keasling

    Article

    Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

    Insert

    GGR
    

    Use

    Tags

    TEV-cleavable His-tag

    Expression

    Bacterial

    Mutation

    SaGGR-I206F-L377H mutant gene

    Promoter

    T7

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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