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pET28b – E. coli RecE residues 564-868

Plasmid

#104534

Purpose

Expresses E. coli RecE residues 564-868

Depositor

Charles Bell

Article

Zhang et al Structure. 2009 May 13;17(5):690-702. do

Insert

RecE residues 564-866 (C-terminal nuclease domain)

Use

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

Plasmid

#91918

Purpose

Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbfCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pC032 - RcdCas13a from Rhodobacter capsulatus DE442

Plasmid

#91922

Purpose

Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcdCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

Plasmid

#91920

Purpose

Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcsCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

Plasmid

#91913

Purpose

Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CaCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-Skp2 promoter-luciferase

Plasmid

#81119

Purpose

Reporter activity of SKP2 gene promoter

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (Skp2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

contains the region from 1_kb upstream of transcr…

Promoter

SKP2

Available since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only
pWZLblasti-CTR1M154A

Plasmid

#53158

Purpose

Expresses human CTR1 M154A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

M154A

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

Plasmid

#91912

Purpose

Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbnCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-I206F-L377H (JBEI-7202)

Plasmid

#110152

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Use

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-I206F-L377H mutant gene

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2 2241-end

Plasmid

#87039

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2 1-1021

Plasmid

#87036

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020

Plasmid

#91911

Purpose

Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbmCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC026 - PpCas13a from Paludibacter propionicigenes WB4

Plasmid

#91916

Purpose

Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

PpCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91914

Purpose

Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CgCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H02

Plasmid

#70956

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Iswi (Iswi Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SZT2 2241-end

Plasmid

#87040

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Promoter

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pGEM-rat Fcepsilon RI gamma

Plasmid

#8375

Depositor

Jean Kinet

Article

Blank et al Nature 1989 Jan 12;337(6203):187-9.

Insert

rat Fc epsilon RI gamma cDNA (Fcer1g Rat)

Use

Beta-gal

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91915

Purpose

Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Cg2Cas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCVL TAL(Nd154C63) SFFV Xba-Sal linker

Plasmid

#50420

Purpose

pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Lentiviral and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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