We narrowed to 11,847 results for: NSI

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pBAD-MtrxRFP2

Plasmid

#172323

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Tags

His-tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTJV1ScGm-rpoB

Plasmid

#166979

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

gentamycin-3-acetyltransferase (aac(3)-Ia )

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-ChPylTMSK

Plasmid

#163915

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pXW101Plux2-Pecf11-gfp

Plasmid

#160823

Purpose

Two-layered AHL-responsive circuit carrying an ECF11-based amplifier

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23101, Plux2, Pecf11

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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MTK4a_024

Plasmid

#123836

Purpose

Encodes Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

ABI

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_026

Plasmid

#123838

Purpose

Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

HA::2xNLS::ABI

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_027

Plasmid

#123839

Purpose

Encodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

HA::2xNLS::GAI

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_022

Plasmid

#123774

Purpose

Encodes Gibberellin Insensitive tagged with his and tagBFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

his::GAI::tagBFP::linker

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_023

Plasmid

#123775

Purpose

Encodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

his::ABI::tagBFP::linker

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_017

Plasmid

#123732

Purpose

Encodes Fluorescent probe for G1/S transition as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Citrine::Geminin(1-110)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_019

Plasmid

#123682

Purpose

Encodes ConL6 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L6-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_020

Plasmid

#123683

Purpose

Encodes ConL7 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L7-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_012

Plasmid

#123675

Purpose

Encodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_015

Plasmid

#123678

Purpose

Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4-LoxP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI126

Plasmid

#126066

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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DeltaNIRD C95S KVS-1

Plasmid

#113068

Purpose

Expresses worm truncated KVS1.2 C113S in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

Insert

C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)

Expression

Mammalian

Mutation

C113S+first 40 AA deletion

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87C448Y

Plasmid

#89329

Purpose

Inducible expression of M87 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP C448Y with 5'UTR

Plasmid

#89325

Purpose

Inducible expression of C448Y spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1C448Y

Plasmid

#89326

Purpose

Inducible expression of M1C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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