We narrowed to 12,431 results for: NSI
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Plasmid#160818PurposeaTc-responsive single-layered circuitDepositorInsertPJ23117-30tetR-t-Ptet2-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterPJ23117, Ptet2Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTSG
Plasmid#159420PurposeTransient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cellsDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-mphR
Plasmid#62570PurposeMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.DepositorInsertmCherry
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
SOX2se-CPG-TV
Plasmid#70155Purposea targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyADepositorInsert5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
UseMouse Targeting and Synthetic BiologyAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTcI42-Wasabi
Plasmid#86118PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42DepositorInsertsTcI42
mWasabi
Tags6xHisExpressionBacterialMutationA67T, K6N, S33T, Y61H, L119P, F122CPromoterpR/pL and pR/pL, LacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL1342
Plasmid#70691PurposeRSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mCherry
Plasmid#68420PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoterDepositorInsertMS2
UseSynthetic BiologyTagsV5 and mCherryExpressionMammalianMutationMS2: V75E/A81GPromoterhuman UbCAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLCS109
Plasmid#209330PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-dTomato-GCaMP6s
Plasmid#99864Purposeexpresses dTomato and GCaMP6s under control of the Tet-off systemDepositorInsertdTomato, GCaMP6s
PromoterTet-responsive promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GFP-aequorin
Plasmid#101011PurposeConstruct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsisPromoterRibiAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
ExpressionBacterialPromoterEc-TTL-P112 and BBa_J23116Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-Vc
Plasmid#183157PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUA66 PcspA GFP No Linker
Plasmid#105605PurposeExpresses GFP under temperature sensitive promoter PcspADepositorInsertGFP
ExpressionBacterialPromoterPcspAAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Vc-C
Plasmid#183158PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCasRx-ccdB
Plasmid#221003PurposeDestination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPPromoterBeta ActinAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/N-Vn
Plasmid#183153PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only