We narrowed to 9,453 results for: Coli

1. pGK401red-­rtetTAK8N, L131L

   Plasmid

   #165968

   Purpose

   Expresses rTetTA (K8N, L131L) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   tetR
   

   Use

   Synthetic Biology

   Tags

   three copies of VP16 (VP48)

   Expression

   Yeast

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-tTA

   Plasmid

   #149363

   Purpose

   AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

   Depositor

   Takeshi Imai

   Article

   Leiwe et al bioRxiv 2022.10.20.512984

   Insert

   tTA2
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB1A3-AD011

   Plasmid

   #116852

   Purpose

   Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   mScarletI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJPC210

   Plasmid

   #234532

   Purpose

   Int13 Recombinase switch; plant T-DNA

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   RUBY
   

   Expression

   Plant

   Mutation

   none

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJPC430

   Plasmid

   #234529

   Purpose

   CinH Recombinase switch; plant T-DNA

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   RUBY
   

   Expression

   Plant

   Mutation

   none

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKL09-GCaMP6f-mScarlet bb118

   Plasmid

   #158979

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-mScarlet fusion
   

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23118 (sigma 70)

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAH69

   Plasmid

   #173782

   Purpose

   The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   HK022 Integrase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMA2641

   Plasmid

   #25435

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   reverse tetracycline-regulated transactivator advanced
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-R1R2-EM7neoPheS

   Plasmid

   #112923

   Purpose

   piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   neoPheS
   

   Use

   Piggybac transposon gateway destination vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKB232

    Plasmid

    #170019

    Purpose

    Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CRAMP
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMYO

    Plasmid

    #34626

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    lpp

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRF-BetI

    Plasmid

    #49357

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    BetI repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKB231

    Plasmid

    #170018

    Purpose

    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Cecropin P1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pADS

    Plasmid

    #19040

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    codon-optimized synthetic amorphadiene synthase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pAdEasy 2-GFP beta-gal

    Plasmid

    #16407

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pNO183

    Plasmid

    #101068

    Purpose

    ppsR2-bphP1 bicistronic operon plasmid

    Depositor

    Jeffrey Tabor

    Article

    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

    Inserts

    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    insert guanine at position 430 to fix frameshift …

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSKrnaSeph

    Plasmid

    #67506

    Purpose

    Express the tRNALys scaffold with the sephadex aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys with sephadex aptamer
    

    Expression

    Bacterial

    Promoter

    lpp

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. p44K

    Plasmid

    #45800

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p007ampGFP

    Plasmid

    #58535

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW357bN

    Plasmid

    #227932

    Purpose

    Accessory plasmid for negative selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII-neg
    T7c
    

    Expression

    Bacterial

    Mutation

    R632S

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTW357d5

    Plasmid

    #227931

    Purpose

    High-stringency accessory plasmid for selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII (recoded)
    T7c
    

    Expression

    Bacterial

    Mutation

    R632S, Q649S

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTK222

    Plasmid

    #13532

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTYT
    

    Tags

    See map

    Expression

    Mammalian

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNM3814

    Plasmid

    #154122

    Purpose

    mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::rtetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGC4593

    Plasmid

    #110206

    Purpose

    Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

    Depositor

    Adam Arkin, Guillaume Cambray

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Inserts

    TEV protease
    aaRS
    

    Expression

    Bacterial

    Promoter

    Pbad and Ptet

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMA3735

    Plasmid

    #184848

    Purpose

    gRNA cloning using blue-white screening for recombinants

    Depositor

    Mikhail Alexeyev

    Article

    Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

    Insert

    LacZalpha
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    alpha-fragment of LacZ

    Promoter

    U6

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAN3944

    Plasmid

    #74702

    Purpose

    Circuit plasmid: 0x1C

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Circuit 0x1C
    

    Expression

    Bacterial

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL11137

    Plasmid

    #191783

    Purpose

    Kanamycin resistance in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    35S::nptII-T-Nos
    

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMARO

    Plasmid

    #178086

    Purpose

    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Insert

    TcR (tetA/tetR)
    

    Expression

    Bacterial

    Mutation

    Tetracycline resistance cassette is flanked by Ec…

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET29b-clbO

    Plasmid

    #114153

    Purpose

    Expresses ClbO

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbO
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFIP

    Plasmid

    #17565

    Depositor

    Jay Keasling

    Article

    Ham et al Biotechnol Bioeng. 2006 May 5. 94(1):1-4

    Insert

    inversion switch
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAN3945

    Plasmid

    #74699

    Purpose

    Circuit plasmid: 0x41

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Circuit 0x41
    

    Expression

    Bacterial

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSWAP_Entry_HAIB

    Plasmid

    #184869

    Purpose

    For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pKS2

    Plasmid

    #24625

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    'tesA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKB222

    Plasmid

    #170012

    Purpose

    Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    LL-37
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSWAP_Entry_H2_beta_GM

    Plasmid

    #184871

    Purpose

    pSwap plasmid part with H2_beta homology arms and gentamycin cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pE_Cre_lambdaCos

    Plasmid

    #173785

    Purpose

    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    Cre
    

    Use

    Cre/Lox and Synthetic Biology

    Mutation

    S102Y and S279Y- please see depositor comments

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SJZ7-KAN

    Plasmid

    #228260

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    kan
    

    Expression

    Yeast

    Promoter

    GPM1 S.c.

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-clbK

    Plasmid

    #114152

    Purpose

    Expresses ClbK

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbK
    

    Tags

    6xhis-Thrombin

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET29b-clbI

    Plasmid

    #114151

    Purpose

    Expresses ClbI

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbI
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET29b-clbF

    Plasmid

    #114148

    Purpose

    Expresses ClbF-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbF
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a-clbC

    Plasmid

    #114145

    Purpose

    Expresses ClbC-nHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbC
    

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET29b-clbE

    Plasmid

    #114147

    Purpose

    Expresses ClbE-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbE
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET29b-clbD

    Plasmid

    #114146

    Purpose

    Expresses ClbD-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbD
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET29b-clbG

    Plasmid

    #114150

    Purpose

    Expresses ClbG

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbG
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pKB230

    Plasmid

    #170017

    Purpose

    Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Protegrin-1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAN4023

    Plasmid

    #74701

    Purpose

    Output plasmid: PBM3R1-YFP

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PBM3R1-YFP
    

    Expression

    Bacterial

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET21-Streptavidin-Dead Aspartate Loop

    Plasmid

    #46368

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Dead Aspartate Loop
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

50. pZH516

    Plasmid

    #102669

    Purpose

    Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge