We narrowed to 22,173 results for: ESC

1. pAAV-CAG-mBlueberry2-P2A-EGFP

   Plasmid

   #184937

   Purpose

   Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mBlueberry2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mKO2-SLBP(18-126)

   Plasmid

   #83914

   Purpose

   Fluorescent probe for S/G2 cell cycle transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mKO2-SLBP(18-126) (SLBP Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PRS_8-jRGECO1a

   Plasmid

   #210399

   Purpose

   AAV-vector for fluorescent calcium measurements in the red spectrum from noradrenergic neurons

   Depositor

   Simon Wiegert

   Article

   Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

   Insert

   jRGeco1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRSx8

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PRS_8-GCaMP8m

   Plasmid

   #210400

   Purpose

   AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

   Depositor

   Simon Wiegert

   Article

   Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

   Insert

   GCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRSx8

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGP-CMV-NES-fRCaMP1

   Plasmid

   #125249

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRCaMp1
   

   Use

   Tags

   NES signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpdCas9 KRAB

   Plasmid

   #188499

   Purpose

   Expresses FLAG tagged SpdCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pShuttle-CMV-iRFP

   Plasmid

   #31856

   Purpose

   a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

   Insert

   iRFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

   Promoter

   CMV

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pvimentin-emiRFP670

   Plasmid

   #136572

   Purpose

   Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   vimentin-emiRFP670
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-iBB-GECO1

   Plasmid

   #194215

   Purpose

   Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

   Depositor

   Jin Zhang

   Article

   Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

   Insert

   iBB-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ERroGFP_iE_C204Q_pCDNA3

    Plasmid

    #48629

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    insertion E147B, C204Q

    Promoter

    Available Since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTubulin-miRFP670nano3

    Plasmid

    #184666

    Purpose

    Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-human alpha-tubulin
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHL-FcHis

    Plasmid

    #99846

    Purpose

    mammalian expression with secretive signal and FC tag

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Fc_His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP-AAV-CAG-iGABASnFR2-WPRE

    Plasmid

    #218883

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-CMV-NEW-fRGECO2

    Plasmid

    #125248

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-SFFV_3C-Twin-Strep

    Plasmid

    #113900

    Purpose

    Mammalian protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETCK3 TGP-E

    Plasmid

    #236255

    Purpose

    Expresses TGP-E, a thermostable fluorescent protein

    Depositor

    Natasha DeVore

    Article

    Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

    Insert

    Thermal Green Protein Q66E (TGP-E)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac operon

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRE135

    Plasmid

    #118489

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2/CMV-Luc/PGK-SB100X

    Plasmid

    #192869

    Purpose

    Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

    Depositor

    Maria Castro, David Largaespada

    Article

    Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

    Inserts

    luc
    SB100X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRE162

    Plasmid

    #118510

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pH2B-emiRFP703

    Plasmid

    #136567

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    H2B-emiRFP703
    

    Use

    Tags

    emiRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA-iLACCO1

    Plasmid

    #218821

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMRE131

    Plasmid

    #118485

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRE137

    Plasmid

    #118491

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRE136

    Plasmid

    #118490

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMRE133

    Plasmid

    #118487

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRE130

    Plasmid

    #118484

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNW-sfGFP(SPS6)

    Plasmid

    #133814

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppta

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pActin-miRFP670nano3

    Plasmid

    #184665

    Purpose

    Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-human beta-actin
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TfR-pHoran4

    Plasmid

    #61547

    Purpose

    Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Use

    Tags

    pHoran4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKD-SepRS-EFSep

    Plasmid

    #34623

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Inserts

    SepRS (MMP0688 Methanocaldococcuc maripaludis)
    EF-Sep (tufB E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac and Ptac (from SepRS- operon like)

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-CAG-DIO-APEX2NES

    Plasmid

    #79907

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    APEX2NES
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pvimentin-emiRFP703

    Plasmid

    #136566

    Purpose

    Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    vimentin-emiRFP703
    

    Use

    Tags

    emiRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA-DiLACCO1

    Plasmid

    #218824

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate control biosensor

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    DiLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQE81L-KOFP7

    Plasmid

    #160330

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2020 May 27;68(21):58

    Insert

    KOFP7
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMRE152

    Plasmid

    #118502

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CAG-Electra2-P2A-EGFP

    Plasmid

    #184936

    Purpose

    Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMRE155

    Plasmid

    #118505

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMRE142

    Plasmid

    #118494

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pemiRFP703-tubulin

    Plasmid

    #136564

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP703-tubulin
    

    Use

    Tags

    emiRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSCMV-H2B-FT-Medium

    Plasmid

    #157670

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Medium-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGBW-m4046828

    Plasmid

    #145742

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pLenti CMV-IFP2.0 T2A HO1

    Plasmid

    #59427

    Purpose

    Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

    Depositor

    Xiaokun Shu

    Article

    Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

    Insert

    IFP2.0T2AHO1 IRES EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pTfR-PATagRFP

    Plasmid

    #31946

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Use

    Tags

    TfR

    Expression

    Mammalian

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    CMV

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCMV-iRFP713-ORP9-PH

    Plasmid

    #214269

    Purpose

    Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    iRFP713-ORP9(1-111) (Osbpl9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCS2+ Lifeact-mCherry

    Plasmid

    #185408

    Purpose

    Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    lifeact
    

    Use

    In vitro synthesis of mrna

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMRE154

    Plasmid

    #118504

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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