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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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V250 pCEP-4HA B56epsilon

Plasmid

#14537

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5C B56 epsilon regulatory subunit of PP2A (PPP2R5E Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
MLV Gag-YFP

Plasmid

#1813

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

YFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and Plac

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only
pTrcAgBIS (CO)

Plasmid

#35153

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pTrc

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-DeAct-SpvB

Plasmid

#89446

Purpose

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Myr-(iSH2-p85)-p110alpha-Myc

Plasmid

#1410

Depositor

Lewis Cantley

Insert

PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

SRalpha promoter

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#82400

Purpose

Gateway-compatible zebrafish elavl3 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

elavl3 promoter

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1 (17)

Plasmid

#26060

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pBR322-TIMER

Plasmid

#103056

Purpose

medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pL

Plasmid

#196286

Purpose

35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV L antigenome encoding codon-optimized L (RdRp)

Use

CRISPR

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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