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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-human-p16INK4A-P2A-mCherry

Plasmid

#137026

Purpose

donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

Depositor

Chunliang Li

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

Insert

p16INK4A-knockin-homology arm-P2A-mCherry

Tags

P2A-mCherry

Expression

Bacterial

Promoter

no promoter

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Linear-GFP

Plasmid

#226258

Purpose

Production of linear RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-CRBNmidi-miniTurbo-3xFLAG

Plasmid

#248675

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo CRBNmidi for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

CRBNmidi

Tags

NLS and miniTurbo-3x FLAG

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2CP-VPg(FCV)

Plasmid

#167314

Purpose

Template pDNA to prepare CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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hBACCS2-IRES-mCherry

Plasmid

#72892

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Mark Guiltinan, Siela Maximova

Article

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Expression

Plant

Promoter

E12-omega

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Plasmid

#85041

Purpose

This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

loxP-stop-loxP

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12i1

Plasmid

#120882

Purpose

Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-mCherry

Plasmid

#175829

Purpose

Express mCherry with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

Plasmid

#162333

Purpose

Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSpCas9-2xVP64

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-TIAM1

Plasmid

#176118

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein promoter-less

Plasmid

#118008

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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TC1154

Plasmid

#164876

Purpose

Split GFP reporter plasmid expressing GFP (b11beta

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta11)

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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TC1541A

Plasmid

#164875

Purpose

Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta1-10)

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSKC11 - UTRQ

Plasmid

#110490

Purpose

Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'-UTR (bp 1-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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