We narrowed to 59,379 results for: %s

1. pAV10.F3

   Plasmid

   #63199

   Purpose

   BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

   Plasmid

   #32520

   Depositor

   Mustafa Sahin

   Article

   Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

   Insert

   Constitutively active Rheb (S16H) (Rheb Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S16H to make constitutively active

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBXNPHM3

   Plasmid

   #110099

   Purpose

   E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VN-beta Synuclein

   Plasmid

   #89483

   Purpose

   expresses human beta Synuclein fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

   Insert

   SNCB (SNCB Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor-tBFP-NLS-Neo (Universal)

   Plasmid

   #80767

   Purpose

   Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET-21a_FnCpf1_2xNLS_protein_expression

   Plasmid

   #124548

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnCpf1_2xNLS
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-hu-N-Myc

   Plasmid

   #50772

   Purpose

   retroviral expression of human N-Myc

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   N-myc (MYCN Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUF1-dCas9-GCN5

   Plasmid

   #122509

   Purpose

   Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Insert

   dCas9-GCN5
   

   Use

   CRISPR

   Tags

   3*FLAG

   Mutation

   dCas9(D10A,H840A)

   Promoter

   PfHsp86

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

    Plasmid

    #167313

    Purpose

    Template pDNA to prepare DD-CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    DD-CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQHA-USP7 WT puroR

    Plasmid

    #46753

    Purpose

    Retroviral vector that expresses wild type HA-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti HRE mutant-Luc pGK Hygro

    Plasmid

    #118707

    Purpose

    reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HREmutant-Luc
    

    Use

    Lentiviral

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQHA-USP11 WT puroR

    Plasmid

    #46749

    Purpose

    Retroviral vector that expresses wild type HA-tagged wild type human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pyc126m

    Plasmid

    #158777

    Purpose

    ribosome-tagged GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN1-CMV-sDarken

    Plasmid

    #184799

    Purpose

    Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    sDarken
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP-S100A10

    Plasmid

    #107200

    Purpose

    Expresses YFP-human S100A10 fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

    Insert

    S100A10 (S100A10 Human)
    

    Tags

    Yellow Fluorescent Protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. meffBlue chromoprotein

    Plasmid

    #117845

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGST2-GST-TEV-OPTN

    Plasmid

    #198428

    Purpose

    Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    optineurin (OPTN Human)
    

    Tags

    GST and TEV

    Expression

    Bacterial

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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