We narrowed to 24,412 results for: lars;

1. Halo-Cyto

   Plasmid

   #124314

   Purpose

   Expresses cytosolic specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Use

   Tags

   Cytoplasm specific seq

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMyosin-miRFP670nano3

   Plasmid

   #184668

   Purpose

   LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-LC-Myosin
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTi4.0_SpdCas9_pmcDA1

   Plasmid

   #204613

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGUS (GB0208)

   Plasmid

   #68203

   Purpose

   Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   GUS coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT2_14_GlyOx

   Plasmid

   #177711

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   pTRX2_5xUAS-ymYPET-tCYC1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-PTDSS1-IRES-Puromycin

   Plasmid

   #134667

   Purpose

   Expresses EGFP-tagged Mitochondria-associated ER membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   PTDSS1 (PTDSS1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPS0377

   Plasmid

   #115509

   Purpose

   Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Constitutive promoter pNeo pOGG001

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHA-mLRH-1

   Plasmid

   #16342

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 May . 25(9):3492-505

   Insert

   LRH-1 (Nr5a2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pCB301TOR-CRISPR

   Plasmid

   #164797

   Purpose

   For gene editing in oomycetes

   Depositor

   Miaoying Tian

   Article

   Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

   Inserts

   PsNLS-hSpCas9
   sgRNA expression cassette
   

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   HAM34 and RPL41

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROP_C9_Puro_EPHA2

    Plasmid

    #183286

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets EPHA2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    EPHA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIG2abc

    Plasmid

    #80617

    Purpose

    pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJBL727

    Plasmid

    #140397

    Purpose

    Expresses CsoR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CsoR-T
    

    Use

    Tags

    6XHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD/HisB-Fast-FT

    Plasmid

    #31916

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmScarlet_NES_C1

    Plasmid

    #85060

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM131

    Plasmid

    #127214

    Purpose

    BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, AtSTM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-UBC-MCP-mNeonGreen

    Plasmid

    #207581

    Purpose

    The lentiviral plasmids for expression of MCP-NeonGreen

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    MS2 coat protein mNeonGreen fusion
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    UBC

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDC216

    Plasmid

    #115791

    Purpose

    Expresses rat Syntaxin 1

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Syntaxin1 (Stx1a Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    C145S S193C C271S C272S

    Promoter

    T7

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmScarlet-I_peroxisome_C1

    Plasmid

    #85065

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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